Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

We determined that the Vitek MS Plus matrix-assisted laser desorption ionization–time of flight mass spectrometry using research-use-only (RUO) v.4.12 and in vitro-diagnostic (IVD) v.3.0 databases accurately identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliens...

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Autores principales: Panagea, Theofano, Pincus, David H., Grogono, Dorothy, Jones, Melissa, Bryant, Josephine, Parkhill, Julian, Floto, R. Andres, Gilligan, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Mycobacteriology and Aerobic Actinomycetes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4473197/
https://www.ncbi.nlm.nih.gov/pubmed/25948607
http://dx.doi.org/10.1128/JCM.00494-15
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author Panagea, Theofano
Pincus, David H.
Grogono, Dorothy
Jones, Melissa
Bryant, Josephine
Parkhill, Julian
Floto, R. Andres
Gilligan, Peter
author_facet Panagea, Theofano
Pincus, David H.
Grogono, Dorothy
Jones, Melissa
Bryant, Josephine
Parkhill, Julian
Floto, R. Andres
Gilligan, Peter
author_sort Panagea, Theofano
collection PubMed
description We determined that the Vitek MS Plus matrix-assisted laser desorption ionization–time of flight mass spectrometry using research-use-only (RUO) v.4.12 and in vitro-diagnostic (IVD) v.3.0 databases accurately identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliense isolates identified by whole-genome sequencing to the species but not the subspecies level, from Middlebrook 7H11 and Burkholderia cepacia selective agars. Peak analysis revealed three peaks potentially able to differentiate between subspecies.
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spelling pubmed-44731972015-07-10 Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Panagea, Theofano Pincus, David H. Grogono, Dorothy Jones, Melissa Bryant, Josephine Parkhill, Julian Floto, R. Andres Gilligan, Peter J Clin Microbiol Mycobacteriology and Aerobic Actinomycetes We determined that the Vitek MS Plus matrix-assisted laser desorption ionization–time of flight mass spectrometry using research-use-only (RUO) v.4.12 and in vitro-diagnostic (IVD) v.3.0 databases accurately identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliense isolates identified by whole-genome sequencing to the species but not the subspecies level, from Middlebrook 7H11 and Burkholderia cepacia selective agars. Peak analysis revealed three peaks potentially able to differentiate between subspecies. American Society for Microbiology 2015-06-18 2015-07 /pmc/articles/PMC4473197/ /pubmed/25948607 http://dx.doi.org/10.1128/JCM.00494-15 Text en Copyright © 2015, Panagea et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Mycobacteriology and Aerobic Actinomycetes
Panagea, Theofano
Pincus, David H.
Grogono, Dorothy
Jones, Melissa
Bryant, Josephine
Parkhill, Julian
Floto, R. Andres
Gilligan, Peter
Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
title Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
title_full Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
title_fullStr Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
title_full_unstemmed Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
title_short Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
title_sort mycobacterium abscessus complex identification with matrix-assisted laser desorption ionization–time of flight mass spectrometry
topic Mycobacteriology and Aerobic Actinomycetes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4473197/
https://www.ncbi.nlm.nih.gov/pubmed/25948607
http://dx.doi.org/10.1128/JCM.00494-15
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