Cargando…

Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)

BACKGROUND: Hexaploid wheat (Triticum aestivum) is a globally important crop. Heat, drought and their combination dramatically reduce wheat yield and quality, but the molecular mechanisms underlying wheat tolerance to extreme environments, especially stress combination, are largely unknown. As an al...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Zhenshan, Xin, Mingming, Qin, Jinxia, Peng, Huiru, Ni, Zhongfu, Yao, Yingyin, Sun, Qixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4474349/
https://www.ncbi.nlm.nih.gov/pubmed/26092253
http://dx.doi.org/10.1186/s12870-015-0511-8
_version_ 1782377259238162432
author Liu, Zhenshan
Xin, Mingming
Qin, Jinxia
Peng, Huiru
Ni, Zhongfu
Yao, Yingyin
Sun, Qixin
author_facet Liu, Zhenshan
Xin, Mingming
Qin, Jinxia
Peng, Huiru
Ni, Zhongfu
Yao, Yingyin
Sun, Qixin
author_sort Liu, Zhenshan
collection PubMed
description BACKGROUND: Hexaploid wheat (Triticum aestivum) is a globally important crop. Heat, drought and their combination dramatically reduce wheat yield and quality, but the molecular mechanisms underlying wheat tolerance to extreme environments, especially stress combination, are largely unknown. As an allohexaploid, wheat consists of three closely related subgenomes (A, B, and D), and was reported to show improved tolerance to stress conditions compared to tetraploid. But so far very little is known about how wheat coordinates the expression of homeologous genes to cope with various environmental constraints on the whole-genome level. RESULTS: To explore the transcriptional response of wheat to the individual and combined stress, we performed high-throughput transcriptome sequencing of seedlings under normal condition and subjected to drought stress (DS), heat stress (HS) and their combination (HD) for 1 h and 6 h, and presented global gene expression reprograms in response to these three stresses. Gene Ontology (GO) enrichment analysis of DS, HS and HD responsive genes revealed an overlap and complexity of functional pathways between each other. Moreover, 4,375 wheat transcription factors were identified on a whole-genome scale based on the released scaffold information by IWGSC, and 1,328 were responsive to stress treatments. Then, the regulatory network analysis of HSFs and DREBs implicated they were both involved in the regulation of DS, HS and HD response and indicated a cross-talk between heat and drought stress. Finally, approximately 68.4 % of homeologous genes were found to exhibit expression partitioning in response to DS, HS or HD, which was further confirmed by using quantitative RT-PCR and Nullisomic-Tetrasomic lines. CONCLUSIONS: A large proportion of wheat homeologs exhibited expression partitioning under normal and abiotic stresses, which possibly contributes to the wide adaptability and distribution of hexaploid wheat in response to various environmental constraints. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0511-8) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4474349
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-44743492015-06-20 Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.) Liu, Zhenshan Xin, Mingming Qin, Jinxia Peng, Huiru Ni, Zhongfu Yao, Yingyin Sun, Qixin BMC Plant Biol Research Article BACKGROUND: Hexaploid wheat (Triticum aestivum) is a globally important crop. Heat, drought and their combination dramatically reduce wheat yield and quality, but the molecular mechanisms underlying wheat tolerance to extreme environments, especially stress combination, are largely unknown. As an allohexaploid, wheat consists of three closely related subgenomes (A, B, and D), and was reported to show improved tolerance to stress conditions compared to tetraploid. But so far very little is known about how wheat coordinates the expression of homeologous genes to cope with various environmental constraints on the whole-genome level. RESULTS: To explore the transcriptional response of wheat to the individual and combined stress, we performed high-throughput transcriptome sequencing of seedlings under normal condition and subjected to drought stress (DS), heat stress (HS) and their combination (HD) for 1 h and 6 h, and presented global gene expression reprograms in response to these three stresses. Gene Ontology (GO) enrichment analysis of DS, HS and HD responsive genes revealed an overlap and complexity of functional pathways between each other. Moreover, 4,375 wheat transcription factors were identified on a whole-genome scale based on the released scaffold information by IWGSC, and 1,328 were responsive to stress treatments. Then, the regulatory network analysis of HSFs and DREBs implicated they were both involved in the regulation of DS, HS and HD response and indicated a cross-talk between heat and drought stress. Finally, approximately 68.4 % of homeologous genes were found to exhibit expression partitioning in response to DS, HS or HD, which was further confirmed by using quantitative RT-PCR and Nullisomic-Tetrasomic lines. CONCLUSIONS: A large proportion of wheat homeologs exhibited expression partitioning under normal and abiotic stresses, which possibly contributes to the wide adaptability and distribution of hexaploid wheat in response to various environmental constraints. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0511-8) contains supplementary material, which is available to authorized users. BioMed Central 2015-06-20 /pmc/articles/PMC4474349/ /pubmed/26092253 http://dx.doi.org/10.1186/s12870-015-0511-8 Text en © Liu et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Zhenshan
Xin, Mingming
Qin, Jinxia
Peng, Huiru
Ni, Zhongfu
Yao, Yingyin
Sun, Qixin
Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)
title Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)
title_full Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)
title_fullStr Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)
title_full_unstemmed Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)
title_short Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)
title_sort temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (triticum aestivum l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4474349/
https://www.ncbi.nlm.nih.gov/pubmed/26092253
http://dx.doi.org/10.1186/s12870-015-0511-8
work_keys_str_mv AT liuzhenshan temporaltranscriptomeprofilingrevealsexpressionpartitioningofhomeologousgenescontributingtoheatanddroughtacclimationinwheattriticumaestivuml
AT xinmingming temporaltranscriptomeprofilingrevealsexpressionpartitioningofhomeologousgenescontributingtoheatanddroughtacclimationinwheattriticumaestivuml
AT qinjinxia temporaltranscriptomeprofilingrevealsexpressionpartitioningofhomeologousgenescontributingtoheatanddroughtacclimationinwheattriticumaestivuml
AT penghuiru temporaltranscriptomeprofilingrevealsexpressionpartitioningofhomeologousgenescontributingtoheatanddroughtacclimationinwheattriticumaestivuml
AT nizhongfu temporaltranscriptomeprofilingrevealsexpressionpartitioningofhomeologousgenescontributingtoheatanddroughtacclimationinwheattriticumaestivuml
AT yaoyingyin temporaltranscriptomeprofilingrevealsexpressionpartitioningofhomeologousgenescontributingtoheatanddroughtacclimationinwheattriticumaestivuml
AT sunqixin temporaltranscriptomeprofilingrevealsexpressionpartitioningofhomeologousgenescontributingtoheatanddroughtacclimationinwheattriticumaestivuml