Cargando…
Compartmentalized Cytokine Responses in Hidradenitis Suppurativa
BACKGROUND: Favorable treatment outcomes with TNF blockade led us to explore cytokine responses in hidradenitis suppurativa (HS). METHODS: Blood monocytes of 120 patients and 24 healthy volunteers were subtyped by flow cytometry. Isolated blood mononuclear cells (PBMCs) were stimulated for cytokine...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4474720/ https://www.ncbi.nlm.nih.gov/pubmed/26091259 http://dx.doi.org/10.1371/journal.pone.0130522 |
_version_ | 1782377327141847040 |
---|---|
author | Kanni, Theodora Tzanetakou, Vassiliki Savva, Athina Kersten, Brigit Pistiki, Aikaterini van de Veerdonk, Frank L. Netea, Mihai G. van der Meer, Jos W. Giamarellos-Bourboulis, Evangelos J. |
author_facet | Kanni, Theodora Tzanetakou, Vassiliki Savva, Athina Kersten, Brigit Pistiki, Aikaterini van de Veerdonk, Frank L. Netea, Mihai G. van der Meer, Jos W. Giamarellos-Bourboulis, Evangelos J. |
author_sort | Kanni, Theodora |
collection | PubMed |
description | BACKGROUND: Favorable treatment outcomes with TNF blockade led us to explore cytokine responses in hidradenitis suppurativa (HS). METHODS: Blood monocytes of 120 patients and 24 healthy volunteers were subtyped by flow cytometry. Isolated blood mononuclear cells (PBMCs) were stimulated for cytokine production; this was repeated in 13 severe patients during treatment with etanercept. Cytokines in pus were measured. RESULTS: CD14(bright)CD16(dim) inflammatory monocytes and patrolling monocytes were increased in Hurley III patients. Cytokine production by stimulated PBMCs was low compared to controls but the cytokine gene copies did not differ, indicating post-translational inhibition. The low production of IL-17 was restored, when cells were incubated with adalimumab. In pus, high concentrations of pro-inflammatory cytokines were detected. Based on the patterns, six different cytokine profiles were discerned, which are potentially relevant for the choice of treatment. Clinical improvement with etanercept was predicted by increased production of IL-1β and IL-17 by PBMCs at week 8. CONCLUSIONS: Findings indicate compartmentalized cytokine expression in HS; high in pus but suppressed in PBMCs. This is modulated through blockade of TNF. |
format | Online Article Text |
id | pubmed-4474720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44747202015-06-30 Compartmentalized Cytokine Responses in Hidradenitis Suppurativa Kanni, Theodora Tzanetakou, Vassiliki Savva, Athina Kersten, Brigit Pistiki, Aikaterini van de Veerdonk, Frank L. Netea, Mihai G. van der Meer, Jos W. Giamarellos-Bourboulis, Evangelos J. PLoS One Research Article BACKGROUND: Favorable treatment outcomes with TNF blockade led us to explore cytokine responses in hidradenitis suppurativa (HS). METHODS: Blood monocytes of 120 patients and 24 healthy volunteers were subtyped by flow cytometry. Isolated blood mononuclear cells (PBMCs) were stimulated for cytokine production; this was repeated in 13 severe patients during treatment with etanercept. Cytokines in pus were measured. RESULTS: CD14(bright)CD16(dim) inflammatory monocytes and patrolling monocytes were increased in Hurley III patients. Cytokine production by stimulated PBMCs was low compared to controls but the cytokine gene copies did not differ, indicating post-translational inhibition. The low production of IL-17 was restored, when cells were incubated with adalimumab. In pus, high concentrations of pro-inflammatory cytokines were detected. Based on the patterns, six different cytokine profiles were discerned, which are potentially relevant for the choice of treatment. Clinical improvement with etanercept was predicted by increased production of IL-1β and IL-17 by PBMCs at week 8. CONCLUSIONS: Findings indicate compartmentalized cytokine expression in HS; high in pus but suppressed in PBMCs. This is modulated through blockade of TNF. Public Library of Science 2015-06-19 /pmc/articles/PMC4474720/ /pubmed/26091259 http://dx.doi.org/10.1371/journal.pone.0130522 Text en © 2015 Kanni et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kanni, Theodora Tzanetakou, Vassiliki Savva, Athina Kersten, Brigit Pistiki, Aikaterini van de Veerdonk, Frank L. Netea, Mihai G. van der Meer, Jos W. Giamarellos-Bourboulis, Evangelos J. Compartmentalized Cytokine Responses in Hidradenitis Suppurativa |
title | Compartmentalized Cytokine Responses in Hidradenitis Suppurativa |
title_full | Compartmentalized Cytokine Responses in Hidradenitis Suppurativa |
title_fullStr | Compartmentalized Cytokine Responses in Hidradenitis Suppurativa |
title_full_unstemmed | Compartmentalized Cytokine Responses in Hidradenitis Suppurativa |
title_short | Compartmentalized Cytokine Responses in Hidradenitis Suppurativa |
title_sort | compartmentalized cytokine responses in hidradenitis suppurativa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4474720/ https://www.ncbi.nlm.nih.gov/pubmed/26091259 http://dx.doi.org/10.1371/journal.pone.0130522 |
work_keys_str_mv | AT kannitheodora compartmentalizedcytokineresponsesinhidradenitissuppurativa AT tzanetakouvassiliki compartmentalizedcytokineresponsesinhidradenitissuppurativa AT savvaathina compartmentalizedcytokineresponsesinhidradenitissuppurativa AT kerstenbrigit compartmentalizedcytokineresponsesinhidradenitissuppurativa AT pistikiaikaterini compartmentalizedcytokineresponsesinhidradenitissuppurativa AT vandeveerdonkfrankl compartmentalizedcytokineresponsesinhidradenitissuppurativa AT neteamihaig compartmentalizedcytokineresponsesinhidradenitissuppurativa AT vandermeerjosw compartmentalizedcytokineresponsesinhidradenitissuppurativa AT giamarellosbourboulisevangelosj compartmentalizedcytokineresponsesinhidradenitissuppurativa |