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Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase

It is well established that the plastidic isoform of glutamine synthetase (GS(2)) is the enzyme in charge of photorespiratory ammonium reassimilation in plants. The metabolic events associated to photorespiratory NH(4) (+) accumulation were analyzed in a Lotus japonicus photorespiratory mutant lacki...

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Autores principales: Pérez-Delgado, Carmen M., García-Calderón, Margarita, Márquez, Antonio J., Betti, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4474828/
https://www.ncbi.nlm.nih.gov/pubmed/26091523
http://dx.doi.org/10.1371/journal.pone.0130438
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author Pérez-Delgado, Carmen M.
García-Calderón, Margarita
Márquez, Antonio J.
Betti, Marco
author_facet Pérez-Delgado, Carmen M.
García-Calderón, Margarita
Márquez, Antonio J.
Betti, Marco
author_sort Pérez-Delgado, Carmen M.
collection PubMed
description It is well established that the plastidic isoform of glutamine synthetase (GS(2)) is the enzyme in charge of photorespiratory ammonium reassimilation in plants. The metabolic events associated to photorespiratory NH(4) (+) accumulation were analyzed in a Lotus japonicus photorespiratory mutant lacking GS(2). The mutant plants accumulated high levels of NH(4) (+) when photorespiration was active, followed by a sudden drop in the levels of this compound. In this paper it was examined the possible existence of enzymatic pathways alternative to GS(2) that could account for this decline in the photorespiratory ammonium. Induction of genes encoding for cytosolic glutamine synthetase (GS(1)), glutamate dehydrogenase (GDH) and asparagine synthetase (ASN) was observed in the mutant in correspondence with the diminishment of NH(4) (+). Measurements of gene expression, polypeptide levels, enzyme activity and metabolite levels were carried out in leaf samples from WT and mutant plants after different periods of time under active photorespiratory conditions. In the case of asparagine synthetase it was not possible to determine enzyme activity and polypeptide content; however, an increased asparagine content in parallel with the induction of ASN gene expression was detected in the mutant plants. This increase in asparagine levels took place concomitantly with an increase in glutamine due to the induction of cytosolic GS(1) in the mutant, thus revealing a major role of cytosolic GS(1) in the reassimilation and detoxification of photorespiratory NH(4) (+) when the plastidic GS(2) isoform is lacking. Moreover, a diminishment in glutamate levels was observed, that may be explained by the induction of NAD(H)-dependent GDH activity.
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spelling pubmed-44748282015-06-30 Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase Pérez-Delgado, Carmen M. García-Calderón, Margarita Márquez, Antonio J. Betti, Marco PLoS One Research Article It is well established that the plastidic isoform of glutamine synthetase (GS(2)) is the enzyme in charge of photorespiratory ammonium reassimilation in plants. The metabolic events associated to photorespiratory NH(4) (+) accumulation were analyzed in a Lotus japonicus photorespiratory mutant lacking GS(2). The mutant plants accumulated high levels of NH(4) (+) when photorespiration was active, followed by a sudden drop in the levels of this compound. In this paper it was examined the possible existence of enzymatic pathways alternative to GS(2) that could account for this decline in the photorespiratory ammonium. Induction of genes encoding for cytosolic glutamine synthetase (GS(1)), glutamate dehydrogenase (GDH) and asparagine synthetase (ASN) was observed in the mutant in correspondence with the diminishment of NH(4) (+). Measurements of gene expression, polypeptide levels, enzyme activity and metabolite levels were carried out in leaf samples from WT and mutant plants after different periods of time under active photorespiratory conditions. In the case of asparagine synthetase it was not possible to determine enzyme activity and polypeptide content; however, an increased asparagine content in parallel with the induction of ASN gene expression was detected in the mutant plants. This increase in asparagine levels took place concomitantly with an increase in glutamine due to the induction of cytosolic GS(1) in the mutant, thus revealing a major role of cytosolic GS(1) in the reassimilation and detoxification of photorespiratory NH(4) (+) when the plastidic GS(2) isoform is lacking. Moreover, a diminishment in glutamate levels was observed, that may be explained by the induction of NAD(H)-dependent GDH activity. Public Library of Science 2015-06-19 /pmc/articles/PMC4474828/ /pubmed/26091523 http://dx.doi.org/10.1371/journal.pone.0130438 Text en © 2015 Pérez-Delgado et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pérez-Delgado, Carmen M.
García-Calderón, Margarita
Márquez, Antonio J.
Betti, Marco
Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase
title Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase
title_full Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase
title_fullStr Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase
title_full_unstemmed Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase
title_short Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase
title_sort reassimilation of photorespiratory ammonium in lotus japonicus plants deficient in plastidic glutamine synthetase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4474828/
https://www.ncbi.nlm.nih.gov/pubmed/26091523
http://dx.doi.org/10.1371/journal.pone.0130438
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