Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation

The established methods for the study of atom-detailed protein structure in the condensed phases, X-ray crystallography and nuclear magnetic resonance spectroscopy, have recently been complemented by new techniques by which nearly or fully desolvated protein structures are probed in gas-phase experi...

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Detalles Bibliográficos
Autores principales: Schennach, Moritz, Breuker, Kathrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Critical Insight
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4475247/
https://www.ncbi.nlm.nih.gov/pubmed/25868904
http://dx.doi.org/10.1007/s13361-015-1088-z
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author Schennach, Moritz
Breuker, Kathrin
author_facet Schennach, Moritz
Breuker, Kathrin
author_sort Schennach, Moritz
collection PubMed
description The established methods for the study of atom-detailed protein structure in the condensed phases, X-ray crystallography and nuclear magnetic resonance spectroscopy, have recently been complemented by new techniques by which nearly or fully desolvated protein structures are probed in gas-phase experiments. Electron capture dissociation (ECD) is unique among these as it provides residue-specific, although indirect, structural information. In this Critical Insight article, we discuss the development of ECD for the structural probing of gaseous protein ions, its potential, and limitations. [Figure: see text]
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spelling pubmed-44752472015-06-24 Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation Schennach, Moritz Breuker, Kathrin J Am Soc Mass Spectrom Critical Insight The established methods for the study of atom-detailed protein structure in the condensed phases, X-ray crystallography and nuclear magnetic resonance spectroscopy, have recently been complemented by new techniques by which nearly or fully desolvated protein structures are probed in gas-phase experiments. Electron capture dissociation (ECD) is unique among these as it provides residue-specific, although indirect, structural information. In this Critical Insight article, we discuss the development of ECD for the structural probing of gaseous protein ions, its potential, and limitations. [Figure: see text] Springer US 2015-04-14 2015 /pmc/articles/PMC4475247/ /pubmed/25868904 http://dx.doi.org/10.1007/s13361-015-1088-z Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Critical Insight
Schennach, Moritz
Breuker, Kathrin
Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation
title Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation
title_full Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation
title_fullStr Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation
title_full_unstemmed Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation
title_short Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation
title_sort probing protein structure and folding in the gas phase by electron capture dissociation
topic Critical Insight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4475247/
https://www.ncbi.nlm.nih.gov/pubmed/25868904
http://dx.doi.org/10.1007/s13361-015-1088-z
work_keys_str_mv AT schennachmoritz probingproteinstructureandfoldinginthegasphasebyelectroncapturedissociation
AT breukerkathrin probingproteinstructureandfoldinginthegasphasebyelectroncapturedissociation

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