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Optimizing a PCR protocol for cpn60-based microbiome profiling of samples variously contaminated with host genomic DNA

BACKGROUND: The current recommended protocol for chaperonin-60 (cpn60) universal target based microbiome profiling includes universal PCR of microbiome samples across an annealing temperature gradient to maximize the diversity of sequences amplified. However, the value of including this gradient app...

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Detalles Bibliográficos
Autores principales:	Johnson, Lisa A, Chaban, Bonnie, Harding, John C S, Hill, Janet E
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4475309/ https://www.ncbi.nlm.nih.gov/pubmed/26092180 http://dx.doi.org/10.1186/s13104-015-1170-4

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