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Gibson assembly: an easy way to clone potyviral full-length infectious cDNA clones expressing an ectopic VPg

BACKGROUND: Approaches to simplify and accelerate the construction of full-length infectious cDNA clones for plant potyviruses have been described, based on cloning strategies involving in vitro ligation or homologous recombination in yeast. In the present study, we developed a faster and more effic...

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Detalles Bibliográficos
Autores principales:	Bordat, Amandine, Houvenaghel, Marie-Christine, German-Retana, Sylvie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4475333/ https://www.ncbi.nlm.nih.gov/pubmed/26070311 http://dx.doi.org/10.1186/s12985-015-0315-3

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