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The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications
Due to the intriguing morphology, lifecycle, and diversity of butterflies and moths, Lepidoptera are emerging as model organisms for the study of genetics, evolution and speciation. The progress of these studies relies on decoding Lepidoptera genomes, both nuclear and mitochondrial. Here we describe...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4475787/ https://www.ncbi.nlm.nih.gov/pubmed/26106582 http://dx.doi.org/10.1016/j.mgene.2015.05.002 |
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author | Shen, Jinhui Cong, Qian Grishin, Nick V. |
author_facet | Shen, Jinhui Cong, Qian Grishin, Nick V. |
author_sort | Shen, Jinhui |
collection | PubMed |
description | Due to the intriguing morphology, lifecycle, and diversity of butterflies and moths, Lepidoptera are emerging as model organisms for the study of genetics, evolution and speciation. The progress of these studies relies on decoding Lepidoptera genomes, both nuclear and mitochondrial. Here we describe a protocol to obtain mitogenomes from Next Generation Sequencing reads performed for whole-genome sequencing and report the complete mitogenome of Papilio (Pterourus) glaucus. The circular mitogenome is 15,306 bp in length and rich in A and T. It contains 13 protein-coding genes (PCGs), 22 transfer-RNA-coding genes (tRNA), and 2 ribosomal-RNA-coding genes (rRNA), with a gene order typical for mitogenomes of Lepidoptera. We performed phylogenetic analyses based on PCG and RNA-coding genes or protein sequences using Bayesian Inference and Maximum Likelihood methods. The phylogenetic trees consistently show that among species with available mitogenomes Papilio glaucus is the closest to Papilio (Agehana) maraho from Asia. |
format | Online Article Text |
id | pubmed-4475787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-44757872015-06-23 The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications Shen, Jinhui Cong, Qian Grishin, Nick V. Meta Gene Article Due to the intriguing morphology, lifecycle, and diversity of butterflies and moths, Lepidoptera are emerging as model organisms for the study of genetics, evolution and speciation. The progress of these studies relies on decoding Lepidoptera genomes, both nuclear and mitochondrial. Here we describe a protocol to obtain mitogenomes from Next Generation Sequencing reads performed for whole-genome sequencing and report the complete mitogenome of Papilio (Pterourus) glaucus. The circular mitogenome is 15,306 bp in length and rich in A and T. It contains 13 protein-coding genes (PCGs), 22 transfer-RNA-coding genes (tRNA), and 2 ribosomal-RNA-coding genes (rRNA), with a gene order typical for mitogenomes of Lepidoptera. We performed phylogenetic analyses based on PCG and RNA-coding genes or protein sequences using Bayesian Inference and Maximum Likelihood methods. The phylogenetic trees consistently show that among species with available mitogenomes Papilio glaucus is the closest to Papilio (Agehana) maraho from Asia. Elsevier 2015-06-14 /pmc/articles/PMC4475787/ /pubmed/26106582 http://dx.doi.org/10.1016/j.mgene.2015.05.002 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Shen, Jinhui Cong, Qian Grishin, Nick V. The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications |
title | The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications |
title_full | The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications |
title_fullStr | The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications |
title_full_unstemmed | The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications |
title_short | The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications |
title_sort | complete mitochondrial genome of papilio glaucus and its phylogenetic implications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4475787/ https://www.ncbi.nlm.nih.gov/pubmed/26106582 http://dx.doi.org/10.1016/j.mgene.2015.05.002 |
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