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Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family
Gene families often consist of members with diverse expression domains reflecting their functions in a wide variety of tissues. However, how the expression of individual members, and thus their tissue-specific functions, diversified during the course of gene family expansion is not well understood....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476152/ https://www.ncbi.nlm.nih.gov/pubmed/25743545 http://dx.doi.org/10.1093/molbev/msv052 |
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author | Tanaka, Kohtaro Diekmann, Yoan Hazbun, Alexis Hijazi, Assia Vreede, Barbara Roch, Fernando Sucena, Élio |
author_facet | Tanaka, Kohtaro Diekmann, Yoan Hazbun, Alexis Hijazi, Assia Vreede, Barbara Roch, Fernando Sucena, Élio |
author_sort | Tanaka, Kohtaro |
collection | PubMed |
description | Gene families often consist of members with diverse expression domains reflecting their functions in a wide variety of tissues. However, how the expression of individual members, and thus their tissue-specific functions, diversified during the course of gene family expansion is not well understood. In this study, we approached this question through the analysis of the duplication history and transcriptional evolution of a rapidly expanding subfamily of insect Ly6 genes. We analyzed different insect genomes and identified seven Ly6 genes that have originated from a single ancestor through sequential duplication within the higher Diptera. We then determined how the original embryonic expression pattern of the founding gene diversified by characterizing its tissue-specific expression in the beetle Tribolium castaneum, the butterfly Bicyclus anynana, and the mosquito Anopheles stephensi and those of its duplicates in three higher dipteran species, representing various stages of the duplication history (Megaselia abdita, Ceratitis capitata, and Drosophila melanogaster). Our results revealed that frequent neofunctionalization episodes contributed to the increased expression breadth of this subfamily and that these events occurred after duplication and speciation events at comparable frequencies. In addition, at each duplication node, we consistently found asymmetric expression divergence. One paralog inherited most of the tissue-specificities of the founder gene, whereas the other paralog evolved drastically reduced expression domains. Our approach attests to the power of combining a well-established duplication history with a comprehensive coverage of representative species in acquiring unequivocal information about the dynamics of gene expression evolution in gene families. |
format | Online Article Text |
id | pubmed-4476152 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44761522015-06-24 Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family Tanaka, Kohtaro Diekmann, Yoan Hazbun, Alexis Hijazi, Assia Vreede, Barbara Roch, Fernando Sucena, Élio Mol Biol Evol Discoveries Gene families often consist of members with diverse expression domains reflecting their functions in a wide variety of tissues. However, how the expression of individual members, and thus their tissue-specific functions, diversified during the course of gene family expansion is not well understood. In this study, we approached this question through the analysis of the duplication history and transcriptional evolution of a rapidly expanding subfamily of insect Ly6 genes. We analyzed different insect genomes and identified seven Ly6 genes that have originated from a single ancestor through sequential duplication within the higher Diptera. We then determined how the original embryonic expression pattern of the founding gene diversified by characterizing its tissue-specific expression in the beetle Tribolium castaneum, the butterfly Bicyclus anynana, and the mosquito Anopheles stephensi and those of its duplicates in three higher dipteran species, representing various stages of the duplication history (Megaselia abdita, Ceratitis capitata, and Drosophila melanogaster). Our results revealed that frequent neofunctionalization episodes contributed to the increased expression breadth of this subfamily and that these events occurred after duplication and speciation events at comparable frequencies. In addition, at each duplication node, we consistently found asymmetric expression divergence. One paralog inherited most of the tissue-specificities of the founder gene, whereas the other paralog evolved drastically reduced expression domains. Our approach attests to the power of combining a well-established duplication history with a comprehensive coverage of representative species in acquiring unequivocal information about the dynamics of gene expression evolution in gene families. Oxford University Press 2015-07 2015-03-04 /pmc/articles/PMC4476152/ /pubmed/25743545 http://dx.doi.org/10.1093/molbev/msv052 Text en © The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Discoveries Tanaka, Kohtaro Diekmann, Yoan Hazbun, Alexis Hijazi, Assia Vreede, Barbara Roch, Fernando Sucena, Élio Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family |
title | Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family |
title_full | Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family |
title_fullStr | Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family |
title_full_unstemmed | Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family |
title_short | Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family |
title_sort | multispecies analysis of expression pattern diversification in the recently expanded insect ly6 gene family |
topic | Discoveries |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476152/ https://www.ncbi.nlm.nih.gov/pubmed/25743545 http://dx.doi.org/10.1093/molbev/msv052 |
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