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Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow

Gene Expression Microarray technology was used to compare oviduct transcriptome between inseminated and non-inseminated pigs during spontaneous oestrus. We used an in vivo model approaching the study from a physiological point of view in which no hormonal treatment (animals were in natural oestrus)...

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Autores principales: López-Úbeda, Rebeca, García-Vázquez, Francisco A., Romar, Raquel, Gadea, Joaquín, Muñoz, Marta, Hunter, Ronald H. F., Coy, Pilar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476686/
https://www.ncbi.nlm.nih.gov/pubmed/26098421
http://dx.doi.org/10.1371/journal.pone.0130128
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author López-Úbeda, Rebeca
García-Vázquez, Francisco A.
Romar, Raquel
Gadea, Joaquín
Muñoz, Marta
Hunter, Ronald H. F.
Coy, Pilar
author_facet López-Úbeda, Rebeca
García-Vázquez, Francisco A.
Romar, Raquel
Gadea, Joaquín
Muñoz, Marta
Hunter, Ronald H. F.
Coy, Pilar
author_sort López-Úbeda, Rebeca
collection PubMed
description Gene Expression Microarray technology was used to compare oviduct transcriptome between inseminated and non-inseminated pigs during spontaneous oestrus. We used an in vivo model approaching the study from a physiological point of view in which no hormonal treatment (animals were in natural oestrus) and no artificial sperm selection (selection was performed within the female genital) were imposed. It is therefore emphasised that no surgical introduction of spermatozoa and no insemination at a site other than the physiological one were used. This approach revealed 17 genes that were two-fold or more up-regulated in oviducts exposed to spermatozoa and/or developing embryos and 9 genes that were two-fold or more down-regulated. Functional analysis of the genes revealed that the top canonical pathways affected by insemination were related to the inflammatory response and immune system (Network 1) to molecular transport, protein trafficking and developmental disorder (Network 2) and to cell-to-cell signalling and interaction (Network 3). Some of the genes in network 1 had been previously detected in the oviduct of human and animals, where they were over-expressed in the presence of spermatozoa or pre-implantation embryos (C3, IGHG1, ITIH4, TNF and SERPINE1) whereas others were not previously reported (SAA2, ALOX12, CD1D and SPP1). Genes in Network 2 included RAB1B and TOR3A, the latter being described for the first time in the oviduct and clearly expressed in the epithelial cells of the mucosa layer. Network 3 integrated the genes with the highest down-regulation level (CYP51, PTH1R and TMOD3). Data in the present study indicate a change in gene expression during gamete encounter at the site of fertilization after a natural sperm selection within the female genital tract. These changes would indicate a modification of the environment preparing the oviduct for a successful fertilization and for an adequate embryo early development.
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spelling pubmed-44766862015-06-25 Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow López-Úbeda, Rebeca García-Vázquez, Francisco A. Romar, Raquel Gadea, Joaquín Muñoz, Marta Hunter, Ronald H. F. Coy, Pilar PLoS One Research Article Gene Expression Microarray technology was used to compare oviduct transcriptome between inseminated and non-inseminated pigs during spontaneous oestrus. We used an in vivo model approaching the study from a physiological point of view in which no hormonal treatment (animals were in natural oestrus) and no artificial sperm selection (selection was performed within the female genital) were imposed. It is therefore emphasised that no surgical introduction of spermatozoa and no insemination at a site other than the physiological one were used. This approach revealed 17 genes that were two-fold or more up-regulated in oviducts exposed to spermatozoa and/or developing embryos and 9 genes that were two-fold or more down-regulated. Functional analysis of the genes revealed that the top canonical pathways affected by insemination were related to the inflammatory response and immune system (Network 1) to molecular transport, protein trafficking and developmental disorder (Network 2) and to cell-to-cell signalling and interaction (Network 3). Some of the genes in network 1 had been previously detected in the oviduct of human and animals, where they were over-expressed in the presence of spermatozoa or pre-implantation embryos (C3, IGHG1, ITIH4, TNF and SERPINE1) whereas others were not previously reported (SAA2, ALOX12, CD1D and SPP1). Genes in Network 2 included RAB1B and TOR3A, the latter being described for the first time in the oviduct and clearly expressed in the epithelial cells of the mucosa layer. Network 3 integrated the genes with the highest down-regulation level (CYP51, PTH1R and TMOD3). Data in the present study indicate a change in gene expression during gamete encounter at the site of fertilization after a natural sperm selection within the female genital tract. These changes would indicate a modification of the environment preparing the oviduct for a successful fertilization and for an adequate embryo early development. Public Library of Science 2015-06-22 /pmc/articles/PMC4476686/ /pubmed/26098421 http://dx.doi.org/10.1371/journal.pone.0130128 Text en © 2015 López-Úbeda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
López-Úbeda, Rebeca
García-Vázquez, Francisco A.
Romar, Raquel
Gadea, Joaquín
Muñoz, Marta
Hunter, Ronald H. F.
Coy, Pilar
Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow
title Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow
title_full Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow
title_fullStr Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow
title_full_unstemmed Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow
title_short Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow
title_sort oviductal transcriptome is modified after insemination during spontaneous ovulation in the sow
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476686/
https://www.ncbi.nlm.nih.gov/pubmed/26098421
http://dx.doi.org/10.1371/journal.pone.0130128
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