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The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities

We tested the ability of the axolotl (Ambystoma mexicanum) fibula to regenerate across segment defects of different size in the absence of intervention or after implant of a unique 8-braid pig small intestine submucosa (SIS) scaffold, with or without incorporated growth factor combinations or tissue...

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Autores principales: Chen, Xiaoping, Song, Fengyu, Jhamb, Deepali, Li, Jiliang, Bottino, Marco C., Palakal, Mathew J., Stocum, David L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476796/
https://www.ncbi.nlm.nih.gov/pubmed/26098852
http://dx.doi.org/10.1371/journal.pone.0130819
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author Chen, Xiaoping
Song, Fengyu
Jhamb, Deepali
Li, Jiliang
Bottino, Marco C.
Palakal, Mathew J.
Stocum, David L.
author_facet Chen, Xiaoping
Song, Fengyu
Jhamb, Deepali
Li, Jiliang
Bottino, Marco C.
Palakal, Mathew J.
Stocum, David L.
author_sort Chen, Xiaoping
collection PubMed
description We tested the ability of the axolotl (Ambystoma mexicanum) fibula to regenerate across segment defects of different size in the absence of intervention or after implant of a unique 8-braid pig small intestine submucosa (SIS) scaffold, with or without incorporated growth factor combinations or tissue protein extract. Fractures and defects of 10% and 20% of the total limb length regenerated well without any intervention, but 40% and 50% defects failed to regenerate after either simple removal of bone or implanting SIS scaffold alone. By contrast, scaffold soaked in the growth factor combination BMP-4/HGF or in protein extract of intact limb tissue promoted partial or extensive induction of cartilage and bone across 50% segment defects in 30%-33% of cases. These results show that BMP-4/HGF and intact tissue protein extract can promote the events required to induce cartilage and bone formation across a segment defect larger than critical size and that the long bones of axolotl limbs are an inexpensive model to screen soluble factors and natural and synthetic scaffolds for their efficacy in stimulating this process.
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spelling pubmed-44767962015-06-25 The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities Chen, Xiaoping Song, Fengyu Jhamb, Deepali Li, Jiliang Bottino, Marco C. Palakal, Mathew J. Stocum, David L. PLoS One Research Article We tested the ability of the axolotl (Ambystoma mexicanum) fibula to regenerate across segment defects of different size in the absence of intervention or after implant of a unique 8-braid pig small intestine submucosa (SIS) scaffold, with or without incorporated growth factor combinations or tissue protein extract. Fractures and defects of 10% and 20% of the total limb length regenerated well without any intervention, but 40% and 50% defects failed to regenerate after either simple removal of bone or implanting SIS scaffold alone. By contrast, scaffold soaked in the growth factor combination BMP-4/HGF or in protein extract of intact limb tissue promoted partial or extensive induction of cartilage and bone across 50% segment defects in 30%-33% of cases. These results show that BMP-4/HGF and intact tissue protein extract can promote the events required to induce cartilage and bone formation across a segment defect larger than critical size and that the long bones of axolotl limbs are an inexpensive model to screen soluble factors and natural and synthetic scaffolds for their efficacy in stimulating this process. Public Library of Science 2015-06-22 /pmc/articles/PMC4476796/ /pubmed/26098852 http://dx.doi.org/10.1371/journal.pone.0130819 Text en © 2015 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Xiaoping
Song, Fengyu
Jhamb, Deepali
Li, Jiliang
Bottino, Marco C.
Palakal, Mathew J.
Stocum, David L.
The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities
title The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities
title_full The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities
title_fullStr The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities
title_full_unstemmed The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities
title_short The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities
title_sort axolotl fibula as a model for the induction of regeneration across large segment defects in long bones of the extremities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476796/
https://www.ncbi.nlm.nih.gov/pubmed/26098852
http://dx.doi.org/10.1371/journal.pone.0130819
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