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In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present st...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477105/ https://www.ncbi.nlm.nih.gov/pubmed/26180533 http://dx.doi.org/10.1155/2015/384267 |
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author | Caxito, Marina L. C. Correia, Rachell R. Gomes, Anne Caroline C. Justo, Graça Coelho, Marsen G. P. Sakuragui, Cássia M. Kuster, Ricardo M. Sabino, Katia C. C. |
author_facet | Caxito, Marina L. C. Correia, Rachell R. Gomes, Anne Caroline C. Justo, Graça Coelho, Marsen G. P. Sakuragui, Cássia M. Kuster, Ricardo M. Sabino, Katia C. C. |
author_sort | Caxito, Marina L. C. |
collection | PubMed |
description | Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy. |
format | Online Article Text |
id | pubmed-4477105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44771052015-07-15 In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract Caxito, Marina L. C. Correia, Rachell R. Gomes, Anne Caroline C. Justo, Graça Coelho, Marsen G. P. Sakuragui, Cássia M. Kuster, Ricardo M. Sabino, Katia C. C. Evid Based Complement Alternat Med Research Article Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy. Hindawi Publishing Corporation 2015 2015-06-09 /pmc/articles/PMC4477105/ /pubmed/26180533 http://dx.doi.org/10.1155/2015/384267 Text en Copyright © 2015 Marina L. C. Caxito et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Caxito, Marina L. C. Correia, Rachell R. Gomes, Anne Caroline C. Justo, Graça Coelho, Marsen G. P. Sakuragui, Cássia M. Kuster, Ricardo M. Sabino, Katia C. C. In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract |
title |
In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract |
title_full |
In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract |
title_fullStr |
In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract |
title_full_unstemmed |
In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract |
title_short |
In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract |
title_sort | in vitro antileukemic activity of xanthosoma sagittifolium (taioba) leaf extract |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477105/ https://www.ncbi.nlm.nih.gov/pubmed/26180533 http://dx.doi.org/10.1155/2015/384267 |
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