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miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development
We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n = 20) and without (n = 29) HPV infection; LSIL (n = 28); HSIL (n = 29); and ICC (n = 8). miRNA expression analysis was performed by comparing the distinct groups with the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477216/ https://www.ncbi.nlm.nih.gov/pubmed/26180794 http://dx.doi.org/10.1155/2015/304584 |
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author | Ribeiro, Joana Marinho-Dias, Joana Monteiro, Paula Loureiro, Joana Baldaque, Inês Medeiros, Rui Sousa, Hugo |
author_facet | Ribeiro, Joana Marinho-Dias, Joana Monteiro, Paula Loureiro, Joana Baldaque, Inês Medeiros, Rui Sousa, Hugo |
author_sort | Ribeiro, Joana |
collection | PubMed |
description | We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n = 20) and without (n = 29) HPV infection; LSIL (n = 28); HSIL (n = 29); and ICC (n = 8). miRNA expression analysis was performed by comparing the distinct groups with the reference group (women with normal epithelium without HPV). For miR-125b, we observed a twofold (2(−ΔΔCt) = 2.11; P = 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2(−ΔΔCt) = 0.21; P = 0.004) in ICC. Similarly, miR-34a expression analysis revealed an increased expression (2(−ΔΔCt) = 1.69; P = 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2(−ΔΔCt) = 2.08; P = 0.042) in ICC. Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity. Results showed that while miR-34a expression seems to be correlated with invasive cervical cancer, miR-125b expression is significantly changed within the different cervical lesions and their levels should be further investigated as possible predictive/prognostic biomarkers using a noninvasive approach. |
format | Online Article Text |
id | pubmed-4477216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44772162015-07-15 miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development Ribeiro, Joana Marinho-Dias, Joana Monteiro, Paula Loureiro, Joana Baldaque, Inês Medeiros, Rui Sousa, Hugo Biomed Res Int Research Article We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n = 20) and without (n = 29) HPV infection; LSIL (n = 28); HSIL (n = 29); and ICC (n = 8). miRNA expression analysis was performed by comparing the distinct groups with the reference group (women with normal epithelium without HPV). For miR-125b, we observed a twofold (2(−ΔΔCt) = 2.11; P = 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2(−ΔΔCt) = 0.21; P = 0.004) in ICC. Similarly, miR-34a expression analysis revealed an increased expression (2(−ΔΔCt) = 1.69; P = 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2(−ΔΔCt) = 2.08; P = 0.042) in ICC. Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity. Results showed that while miR-34a expression seems to be correlated with invasive cervical cancer, miR-125b expression is significantly changed within the different cervical lesions and their levels should be further investigated as possible predictive/prognostic biomarkers using a noninvasive approach. Hindawi Publishing Corporation 2015 2015-06-09 /pmc/articles/PMC4477216/ /pubmed/26180794 http://dx.doi.org/10.1155/2015/304584 Text en Copyright © 2015 Joana Ribeiro et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ribeiro, Joana Marinho-Dias, Joana Monteiro, Paula Loureiro, Joana Baldaque, Inês Medeiros, Rui Sousa, Hugo miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development |
title | miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development |
title_full | miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development |
title_fullStr | miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development |
title_full_unstemmed | miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development |
title_short | miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development |
title_sort | mir-34a and mir-125b expression in hpv infection and cervical cancer development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477216/ https://www.ncbi.nlm.nih.gov/pubmed/26180794 http://dx.doi.org/10.1155/2015/304584 |
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