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SUMO-Specific Protease 2 (SENP2) Is an Important Regulator of Fatty Acid Metabolism in Skeletal Muscle

Small ubiquitin-like modifier (SUMO)-specific proteases (SENPs) that reverse protein modification by SUMO are involved in the control of numerous cellular processes, including transcription, cell division, and cancer development. However, the physiological function of SENPs in energy metabolism rema...

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Autores principales: Koo, Young Do, Choi, Jin Woo, Kim, Myungjin, Chae, Sehyun, Ahn, Byung Yong, Kim, Min, Oh, Byung Chul, Hwang, Daehee, Seol, Jae Hong, Kim, Young-Bum, Park, Young Joo, Chung, Sung Soo, Park, Kyong Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Diabetes Association 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477359/
https://www.ncbi.nlm.nih.gov/pubmed/25784542
http://dx.doi.org/10.2337/db15-0115
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author Koo, Young Do
Choi, Jin Woo
Kim, Myungjin
Chae, Sehyun
Ahn, Byung Yong
Kim, Min
Oh, Byung Chul
Hwang, Daehee
Seol, Jae Hong
Kim, Young-Bum
Park, Young Joo
Chung, Sung Soo
Park, Kyong Soo
author_facet Koo, Young Do
Choi, Jin Woo
Kim, Myungjin
Chae, Sehyun
Ahn, Byung Yong
Kim, Min
Oh, Byung Chul
Hwang, Daehee
Seol, Jae Hong
Kim, Young-Bum
Park, Young Joo
Chung, Sung Soo
Park, Kyong Soo
author_sort Koo, Young Do
collection PubMed
description Small ubiquitin-like modifier (SUMO)-specific proteases (SENPs) that reverse protein modification by SUMO are involved in the control of numerous cellular processes, including transcription, cell division, and cancer development. However, the physiological function of SENPs in energy metabolism remains unclear. Here, we investigated the role of SENP2 in fatty acid metabolism in C2C12 myotubes and in vivo. In C2C12 myotubes, treatment with saturated fatty acids, like palmitate, led to nuclear factor-κB–mediated increase in the expression of SENP2. This increase promoted the recruitment of peroxisome proliferator–activated receptor (PPAR)δ and PPARγ, through desumoylation of PPARs, to the promoters of the genes involved in fatty acid oxidation (FAO), such as carnitine-palmitoyl transferase-1 (CPT1b) and long-chain acyl-CoA synthetase 1 (ACSL1). In addition, SENP2 overexpression substantially increased FAO in C2C12 myotubes. Consistent with the cell culture system, muscle-specific SENP2 overexpression led to a marked increase in the mRNA levels of CPT1b and ACSL1 and thereby in FAO in the skeletal muscle, which ultimately alleviated high-fat diet–induced obesity and insulin resistance. Collectively, these data identify SENP2 as an important regulator of fatty acid metabolism in skeletal muscle and further implicate that muscle SENP2 could be a novel therapeutic target for the treatment of obesity-linked metabolic disorders.
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spelling pubmed-44773592016-07-01 SUMO-Specific Protease 2 (SENP2) Is an Important Regulator of Fatty Acid Metabolism in Skeletal Muscle Koo, Young Do Choi, Jin Woo Kim, Myungjin Chae, Sehyun Ahn, Byung Yong Kim, Min Oh, Byung Chul Hwang, Daehee Seol, Jae Hong Kim, Young-Bum Park, Young Joo Chung, Sung Soo Park, Kyong Soo Diabetes Metabolism Small ubiquitin-like modifier (SUMO)-specific proteases (SENPs) that reverse protein modification by SUMO are involved in the control of numerous cellular processes, including transcription, cell division, and cancer development. However, the physiological function of SENPs in energy metabolism remains unclear. Here, we investigated the role of SENP2 in fatty acid metabolism in C2C12 myotubes and in vivo. In C2C12 myotubes, treatment with saturated fatty acids, like palmitate, led to nuclear factor-κB–mediated increase in the expression of SENP2. This increase promoted the recruitment of peroxisome proliferator–activated receptor (PPAR)δ and PPARγ, through desumoylation of PPARs, to the promoters of the genes involved in fatty acid oxidation (FAO), such as carnitine-palmitoyl transferase-1 (CPT1b) and long-chain acyl-CoA synthetase 1 (ACSL1). In addition, SENP2 overexpression substantially increased FAO in C2C12 myotubes. Consistent with the cell culture system, muscle-specific SENP2 overexpression led to a marked increase in the mRNA levels of CPT1b and ACSL1 and thereby in FAO in the skeletal muscle, which ultimately alleviated high-fat diet–induced obesity and insulin resistance. Collectively, these data identify SENP2 as an important regulator of fatty acid metabolism in skeletal muscle and further implicate that muscle SENP2 could be a novel therapeutic target for the treatment of obesity-linked metabolic disorders. American Diabetes Association 2015-07 2015-03-17 /pmc/articles/PMC4477359/ /pubmed/25784542 http://dx.doi.org/10.2337/db15-0115 Text en © 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.
spellingShingle Metabolism
Koo, Young Do
Choi, Jin Woo
Kim, Myungjin
Chae, Sehyun
Ahn, Byung Yong
Kim, Min
Oh, Byung Chul
Hwang, Daehee
Seol, Jae Hong
Kim, Young-Bum
Park, Young Joo
Chung, Sung Soo
Park, Kyong Soo
SUMO-Specific Protease 2 (SENP2) Is an Important Regulator of Fatty Acid Metabolism in Skeletal Muscle
title SUMO-Specific Protease 2 (SENP2) Is an Important Regulator of Fatty Acid Metabolism in Skeletal Muscle
title_full SUMO-Specific Protease 2 (SENP2) Is an Important Regulator of Fatty Acid Metabolism in Skeletal Muscle
title_fullStr SUMO-Specific Protease 2 (SENP2) Is an Important Regulator of Fatty Acid Metabolism in Skeletal Muscle
title_full_unstemmed SUMO-Specific Protease 2 (SENP2) Is an Important Regulator of Fatty Acid Metabolism in Skeletal Muscle
title_short SUMO-Specific Protease 2 (SENP2) Is an Important Regulator of Fatty Acid Metabolism in Skeletal Muscle
title_sort sumo-specific protease 2 (senp2) is an important regulator of fatty acid metabolism in skeletal muscle
topic Metabolism
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477359/
https://www.ncbi.nlm.nih.gov/pubmed/25784542
http://dx.doi.org/10.2337/db15-0115
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