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Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains

Mycobacterium colombiense is a novel member of the Mycobacterium avium complex, which produces respiratory and disseminated infections in immunosuppressed patients. Currently, the morphological and genetic bases underlying the phenotypic features of M. colombiense strains remain unknown. In the pres...

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Autores principales: Maya-Hoyos, Milena, Leguizamón, John, Mariño-Ramírez, Leonardo, Soto, Carlos Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477443/
https://www.ncbi.nlm.nih.gov/pubmed/26180799
http://dx.doi.org/10.1155/2015/419549
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author Maya-Hoyos, Milena
Leguizamón, John
Mariño-Ramírez, Leonardo
Soto, Carlos Y.
author_facet Maya-Hoyos, Milena
Leguizamón, John
Mariño-Ramírez, Leonardo
Soto, Carlos Y.
author_sort Maya-Hoyos, Milena
collection PubMed
description Mycobacterium colombiense is a novel member of the Mycobacterium avium complex, which produces respiratory and disseminated infections in immunosuppressed patients. Currently, the morphological and genetic bases underlying the phenotypic features of M. colombiense strains remain unknown. In the present study, we demonstrated that M. colombiense strains displaying smooth morphology show increased biofilm formation on hydrophobic surfaces and sliding on motility plates. Thin-layer chromatography experiments showed that M. colombiense strains displaying smooth colonies produce large amounts of glycolipids with a chromatographic behaviour similar to that of the glycopeptidolipids (GPLs) of M. avium. Conversely, we observed a natural rough variant of M. colombiense (57B strain) lacking pigmentation and exhibiting impaired sliding, biofilm formation, and GPL production. Bioinformatics analyses revealed a gene cluster that is likely involved in GPL biosynthesis in M. colombiense CECT 3035. RT-qPCR experiments showed that motile culture conditions activate the transcription of genes possibly involved in key enzymatic activities of GPL biosynthesis.
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spelling pubmed-44774432015-07-15 Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains Maya-Hoyos, Milena Leguizamón, John Mariño-Ramírez, Leonardo Soto, Carlos Y. Biomed Res Int Research Article Mycobacterium colombiense is a novel member of the Mycobacterium avium complex, which produces respiratory and disseminated infections in immunosuppressed patients. Currently, the morphological and genetic bases underlying the phenotypic features of M. colombiense strains remain unknown. In the present study, we demonstrated that M. colombiense strains displaying smooth morphology show increased biofilm formation on hydrophobic surfaces and sliding on motility plates. Thin-layer chromatography experiments showed that M. colombiense strains displaying smooth colonies produce large amounts of glycolipids with a chromatographic behaviour similar to that of the glycopeptidolipids (GPLs) of M. avium. Conversely, we observed a natural rough variant of M. colombiense (57B strain) lacking pigmentation and exhibiting impaired sliding, biofilm formation, and GPL production. Bioinformatics analyses revealed a gene cluster that is likely involved in GPL biosynthesis in M. colombiense CECT 3035. RT-qPCR experiments showed that motile culture conditions activate the transcription of genes possibly involved in key enzymatic activities of GPL biosynthesis. Hindawi Publishing Corporation 2015 2015-05-28 /pmc/articles/PMC4477443/ /pubmed/26180799 http://dx.doi.org/10.1155/2015/419549 Text en Copyright © 2015 Milena Maya-Hoyos et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Maya-Hoyos, Milena
Leguizamón, John
Mariño-Ramírez, Leonardo
Soto, Carlos Y.
Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains
title Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains
title_full Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains
title_fullStr Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains
title_full_unstemmed Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains
title_short Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains
title_sort sliding motility, biofilm formation, and glycopeptidolipid production in mycobacterium colombiense strains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477443/
https://www.ncbi.nlm.nih.gov/pubmed/26180799
http://dx.doi.org/10.1155/2015/419549
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