Intramolecular circularization increases efficiency of RNA sequencing and enables CLIP-Seq of nuclear RNA from human cells

RNA sequencing (RNA-Seq) is a powerful tool for analyzing the identity of cellular RNAs but is often limited by the amount of material available for analysis. In spite of extensive efforts employing existing protocols, we observed that it was not possible to obtain useful sequencing libraries from n...

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Detalles Bibliográficos
Autores principales: Chu, Yongjun, Wang, Tao, Dodd, David, Xie, Yang, Janowski, Bethany A., Corey, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477644/
https://www.ncbi.nlm.nih.gov/pubmed/25813040
http://dx.doi.org/10.1093/nar/gkv213

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477644/
https://www.ncbi.nlm.nih.gov/pubmed/25813040
http://dx.doi.org/10.1093/nar/gkv213

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