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Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study
An alcohol use disorder (AUD) is associated with an increased susceptibility to respiratory infection and injury and, upon hospitalization, higher mortality rates. Studies in model systems show effects of alcohol on mitochondrial function, lipid metabolism and antioxidant systems. The present study...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477879/ https://www.ncbi.nlm.nih.gov/pubmed/26102199 http://dx.doi.org/10.1371/journal.pone.0129570 |
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author | Obianyo, Obiamaka Liang, Yan Burnham, Ellen L. Mehta, Ashish Park, Youngja Uppal, Karan Harris, Frank L. Jones, Dean P. Brown, Lou Ann S. |
author_facet | Obianyo, Obiamaka Liang, Yan Burnham, Ellen L. Mehta, Ashish Park, Youngja Uppal, Karan Harris, Frank L. Jones, Dean P. Brown, Lou Ann S. |
author_sort | Obianyo, Obiamaka |
collection | PubMed |
description | An alcohol use disorder (AUD) is associated with an increased susceptibility to respiratory infection and injury and, upon hospitalization, higher mortality rates. Studies in model systems show effects of alcohol on mitochondrial function, lipid metabolism and antioxidant systems. The present study applied high-resolution metabolomics to test for these changes in bronchoalveolar lavage fluid (BALF) of subjects with an AUD. Smokers were excluded to avoid confounding effects and compliance was verified by cotinine measurements. Statistically significant metabolic features, differentially expressed by control and AUD subjects, were identified by statistical and bioinformatic methods. The results show that fatty acid and acylcarnitine concentrations were increased in AUD subjects, consistent with perturbed mitochondrial and lipid metabolism. Decreased concentrations of methyl-donor compounds suggest altered one-carbon metabolism and oxidative stress. An accumulation of peptides suggests proteolytic activity, which could reflect altered epithelial barrier function. Two metabolites of possible microbial origin suggest subclinical bacterial infection. Furthermore, increased diacetylspermine suggests additional metabolic perturbations, which could contribute to dysregulated alveolar macrophage function and vulnerability to infection. Together, the results show an extended metabolic consequence of AUD in the bronchoalveolar space. |
format | Online Article Text |
id | pubmed-4477879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44778792015-07-02 Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study Obianyo, Obiamaka Liang, Yan Burnham, Ellen L. Mehta, Ashish Park, Youngja Uppal, Karan Harris, Frank L. Jones, Dean P. Brown, Lou Ann S. PLoS One Research Article An alcohol use disorder (AUD) is associated with an increased susceptibility to respiratory infection and injury and, upon hospitalization, higher mortality rates. Studies in model systems show effects of alcohol on mitochondrial function, lipid metabolism and antioxidant systems. The present study applied high-resolution metabolomics to test for these changes in bronchoalveolar lavage fluid (BALF) of subjects with an AUD. Smokers were excluded to avoid confounding effects and compliance was verified by cotinine measurements. Statistically significant metabolic features, differentially expressed by control and AUD subjects, were identified by statistical and bioinformatic methods. The results show that fatty acid and acylcarnitine concentrations were increased in AUD subjects, consistent with perturbed mitochondrial and lipid metabolism. Decreased concentrations of methyl-donor compounds suggest altered one-carbon metabolism and oxidative stress. An accumulation of peptides suggests proteolytic activity, which could reflect altered epithelial barrier function. Two metabolites of possible microbial origin suggest subclinical bacterial infection. Furthermore, increased diacetylspermine suggests additional metabolic perturbations, which could contribute to dysregulated alveolar macrophage function and vulnerability to infection. Together, the results show an extended metabolic consequence of AUD in the bronchoalveolar space. Public Library of Science 2015-06-23 /pmc/articles/PMC4477879/ /pubmed/26102199 http://dx.doi.org/10.1371/journal.pone.0129570 Text en © 2015 Obianyo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Obianyo, Obiamaka Liang, Yan Burnham, Ellen L. Mehta, Ashish Park, Youngja Uppal, Karan Harris, Frank L. Jones, Dean P. Brown, Lou Ann S. Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study |
title | Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study |
title_full | Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study |
title_fullStr | Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study |
title_full_unstemmed | Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study |
title_short | Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study |
title_sort | metabolic consequences of chronic alcohol abuse in non-smokers: a pilot study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477879/ https://www.ncbi.nlm.nih.gov/pubmed/26102199 http://dx.doi.org/10.1371/journal.pone.0129570 |
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