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Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study

An alcohol use disorder (AUD) is associated with an increased susceptibility to respiratory infection and injury and, upon hospitalization, higher mortality rates. Studies in model systems show effects of alcohol on mitochondrial function, lipid metabolism and antioxidant systems. The present study...

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Autores principales: Obianyo, Obiamaka, Liang, Yan, Burnham, Ellen L., Mehta, Ashish, Park, Youngja, Uppal, Karan, Harris, Frank L., Jones, Dean P., Brown, Lou Ann S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477879/
https://www.ncbi.nlm.nih.gov/pubmed/26102199
http://dx.doi.org/10.1371/journal.pone.0129570
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author Obianyo, Obiamaka
Liang, Yan
Burnham, Ellen L.
Mehta, Ashish
Park, Youngja
Uppal, Karan
Harris, Frank L.
Jones, Dean P.
Brown, Lou Ann S.
author_facet Obianyo, Obiamaka
Liang, Yan
Burnham, Ellen L.
Mehta, Ashish
Park, Youngja
Uppal, Karan
Harris, Frank L.
Jones, Dean P.
Brown, Lou Ann S.
author_sort Obianyo, Obiamaka
collection PubMed
description An alcohol use disorder (AUD) is associated with an increased susceptibility to respiratory infection and injury and, upon hospitalization, higher mortality rates. Studies in model systems show effects of alcohol on mitochondrial function, lipid metabolism and antioxidant systems. The present study applied high-resolution metabolomics to test for these changes in bronchoalveolar lavage fluid (BALF) of subjects with an AUD. Smokers were excluded to avoid confounding effects and compliance was verified by cotinine measurements. Statistically significant metabolic features, differentially expressed by control and AUD subjects, were identified by statistical and bioinformatic methods. The results show that fatty acid and acylcarnitine concentrations were increased in AUD subjects, consistent with perturbed mitochondrial and lipid metabolism. Decreased concentrations of methyl-donor compounds suggest altered one-carbon metabolism and oxidative stress. An accumulation of peptides suggests proteolytic activity, which could reflect altered epithelial barrier function. Two metabolites of possible microbial origin suggest subclinical bacterial infection. Furthermore, increased diacetylspermine suggests additional metabolic perturbations, which could contribute to dysregulated alveolar macrophage function and vulnerability to infection. Together, the results show an extended metabolic consequence of AUD in the bronchoalveolar space.
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spelling pubmed-44778792015-07-02 Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study Obianyo, Obiamaka Liang, Yan Burnham, Ellen L. Mehta, Ashish Park, Youngja Uppal, Karan Harris, Frank L. Jones, Dean P. Brown, Lou Ann S. PLoS One Research Article An alcohol use disorder (AUD) is associated with an increased susceptibility to respiratory infection and injury and, upon hospitalization, higher mortality rates. Studies in model systems show effects of alcohol on mitochondrial function, lipid metabolism and antioxidant systems. The present study applied high-resolution metabolomics to test for these changes in bronchoalveolar lavage fluid (BALF) of subjects with an AUD. Smokers were excluded to avoid confounding effects and compliance was verified by cotinine measurements. Statistically significant metabolic features, differentially expressed by control and AUD subjects, were identified by statistical and bioinformatic methods. The results show that fatty acid and acylcarnitine concentrations were increased in AUD subjects, consistent with perturbed mitochondrial and lipid metabolism. Decreased concentrations of methyl-donor compounds suggest altered one-carbon metabolism and oxidative stress. An accumulation of peptides suggests proteolytic activity, which could reflect altered epithelial barrier function. Two metabolites of possible microbial origin suggest subclinical bacterial infection. Furthermore, increased diacetylspermine suggests additional metabolic perturbations, which could contribute to dysregulated alveolar macrophage function and vulnerability to infection. Together, the results show an extended metabolic consequence of AUD in the bronchoalveolar space. Public Library of Science 2015-06-23 /pmc/articles/PMC4477879/ /pubmed/26102199 http://dx.doi.org/10.1371/journal.pone.0129570 Text en © 2015 Obianyo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Obianyo, Obiamaka
Liang, Yan
Burnham, Ellen L.
Mehta, Ashish
Park, Youngja
Uppal, Karan
Harris, Frank L.
Jones, Dean P.
Brown, Lou Ann S.
Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study
title Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study
title_full Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study
title_fullStr Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study
title_full_unstemmed Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study
title_short Metabolic Consequences of Chronic Alcohol Abuse in Non-Smokers: A Pilot Study
title_sort metabolic consequences of chronic alcohol abuse in non-smokers: a pilot study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477879/
https://www.ncbi.nlm.nih.gov/pubmed/26102199
http://dx.doi.org/10.1371/journal.pone.0129570
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