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EnD-Seq and AppEnD: sequencing 3′ ends to identify nontemplated tails and degradation intermediates
Existing methods for detecting RNA intermediates resulting from exonuclease degradation are low-throughput and laborious. In addition, mapping the 3′ ends of RNA molecules to the genome after high-throughput sequencing is challenging, particularly if the 3′ ends contain post-transcriptional modifica...
Autores principales: | Welch, Joshua D., Slevin, Michael K., Tatomer, Deirdre C., Duronio, Robert J., Prins, Jan F., Marzluff, William F. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478355/ https://www.ncbi.nlm.nih.gov/pubmed/26015596 http://dx.doi.org/10.1261/rna.048785.114 |
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