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X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads
X chromosome dosage compensation is required for male viability in Drosophila. Dosage compensation relative to autosomes is two-fold, but this is likely to be due to a combination of homeostatic gene-by-gene regulation and chromosome-wide regulation. We have baseline values for gene-by-gene dosage c...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478536/ https://www.ncbi.nlm.nih.gov/pubmed/25850426 http://dx.doi.org/10.1534/g3.115.017632 |
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author | Chen, Zhen-Xia Oliver, Brian |
author_facet | Chen, Zhen-Xia Oliver, Brian |
author_sort | Chen, Zhen-Xia |
collection | PubMed |
description | X chromosome dosage compensation is required for male viability in Drosophila. Dosage compensation relative to autosomes is two-fold, but this is likely to be due to a combination of homeostatic gene-by-gene regulation and chromosome-wide regulation. We have baseline values for gene-by-gene dosage compensation on autosomes, but not for the X chromosome. Given the evolutionary history of sex chromosomes, these baseline values could differ. We used a series of deficiencies on the X and autosomes, along with mutations in the sex-determination gene transformer-2, to carefully measure the sex-independent X-chromosome response to gene dosage in adult heads by RNA sequencing. We observed modest and indistinguishable dosage compensation for both X chromosome and autosome genes, suggesting that the X chromosome is neither inherently more robust nor sensitive to dosage change. |
format | Online Article Text |
id | pubmed-4478536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-44785362015-06-29 X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads Chen, Zhen-Xia Oliver, Brian G3 (Bethesda) Genetics of Sex X chromosome dosage compensation is required for male viability in Drosophila. Dosage compensation relative to autosomes is two-fold, but this is likely to be due to a combination of homeostatic gene-by-gene regulation and chromosome-wide regulation. We have baseline values for gene-by-gene dosage compensation on autosomes, but not for the X chromosome. Given the evolutionary history of sex chromosomes, these baseline values could differ. We used a series of deficiencies on the X and autosomes, along with mutations in the sex-determination gene transformer-2, to carefully measure the sex-independent X-chromosome response to gene dosage in adult heads by RNA sequencing. We observed modest and indistinguishable dosage compensation for both X chromosome and autosome genes, suggesting that the X chromosome is neither inherently more robust nor sensitive to dosage change. Genetics Society of America 2015-04-07 /pmc/articles/PMC4478536/ /pubmed/25850426 http://dx.doi.org/10.1534/g3.115.017632 Text en Copyright © 2015 Chen and Oliver http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genetics of Sex Chen, Zhen-Xia Oliver, Brian X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads |
title | X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads |
title_full | X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads |
title_fullStr | X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads |
title_full_unstemmed | X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads |
title_short | X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads |
title_sort | x chromosome and autosome dosage responses in drosophila melanogaster heads |
topic | Genetics of Sex |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478536/ https://www.ncbi.nlm.nih.gov/pubmed/25850426 http://dx.doi.org/10.1534/g3.115.017632 |
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