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The oncolytic effects of reovirus in canine solid tumor cell lines

Oncolytic virotherapy is a new strategy for cancer treatment for humans and dogs. Reovirus has been proven to be a potent oncolytic virus in human medicine. Our laboratory has previously reported that canine mast cell tumor and canine lymphoma were susceptible to reovirus. In this study, canine soli...

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Autores principales: IGASE, Masaya, HWANG, Chung Chew, COFFEY, Matt, OKUDA, Masaru, NOGUCHI, Shunsuke, MIZUNO, Takuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478733/
https://www.ncbi.nlm.nih.gov/pubmed/25648933
http://dx.doi.org/10.1292/jvms.14-0570
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author IGASE, Masaya
HWANG, Chung Chew
COFFEY, Matt
OKUDA, Masaru
NOGUCHI, Shunsuke
MIZUNO, Takuya
author_facet IGASE, Masaya
HWANG, Chung Chew
COFFEY, Matt
OKUDA, Masaru
NOGUCHI, Shunsuke
MIZUNO, Takuya
author_sort IGASE, Masaya
collection PubMed
description Oncolytic virotherapy is a new strategy for cancer treatment for humans and dogs. Reovirus has been proven to be a potent oncolytic virus in human medicine. Our laboratory has previously reported that canine mast cell tumor and canine lymphoma were susceptible to reovirus. In this study, canine solid tumor cell lines (mammary gland tumor, osteosarcoma and malignant melanoma) were tested to determine their susceptibility towards reovirus. We demonstrated that reovirus induces more than 50% cell death in three canine mammary gland tumors and one canine malignant melanoma cell line. The reovirus-induced cell death occurred via the activation of caspase 3. Ras activation has been shown to be one of the important mechanisms of reovirus-susceptibility in human cancers. However, Ras activation was not related to the reovirus-susceptibility in canine solid tumor cell lines, which was similar to reports in canine mast cell tumor and canine lymphoma. The results of this study highly suggest that canine mammary gland tumor and canine malignant melanoma are also potential candidates for reovirus therapy in veterinary oncology.
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spelling pubmed-44787332015-06-24 The oncolytic effects of reovirus in canine solid tumor cell lines IGASE, Masaya HWANG, Chung Chew COFFEY, Matt OKUDA, Masaru NOGUCHI, Shunsuke MIZUNO, Takuya J Vet Med Sci Internal Medicine Oncolytic virotherapy is a new strategy for cancer treatment for humans and dogs. Reovirus has been proven to be a potent oncolytic virus in human medicine. Our laboratory has previously reported that canine mast cell tumor and canine lymphoma were susceptible to reovirus. In this study, canine solid tumor cell lines (mammary gland tumor, osteosarcoma and malignant melanoma) were tested to determine their susceptibility towards reovirus. We demonstrated that reovirus induces more than 50% cell death in three canine mammary gland tumors and one canine malignant melanoma cell line. The reovirus-induced cell death occurred via the activation of caspase 3. Ras activation has been shown to be one of the important mechanisms of reovirus-susceptibility in human cancers. However, Ras activation was not related to the reovirus-susceptibility in canine solid tumor cell lines, which was similar to reports in canine mast cell tumor and canine lymphoma. The results of this study highly suggest that canine mammary gland tumor and canine malignant melanoma are also potential candidates for reovirus therapy in veterinary oncology. The Japanese Society of Veterinary Science 2015-01-16 2015-05 /pmc/articles/PMC4478733/ /pubmed/25648933 http://dx.doi.org/10.1292/jvms.14-0570 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Internal Medicine
IGASE, Masaya
HWANG, Chung Chew
COFFEY, Matt
OKUDA, Masaru
NOGUCHI, Shunsuke
MIZUNO, Takuya
The oncolytic effects of reovirus in canine solid tumor cell lines
title The oncolytic effects of reovirus in canine solid tumor cell lines
title_full The oncolytic effects of reovirus in canine solid tumor cell lines
title_fullStr The oncolytic effects of reovirus in canine solid tumor cell lines
title_full_unstemmed The oncolytic effects of reovirus in canine solid tumor cell lines
title_short The oncolytic effects of reovirus in canine solid tumor cell lines
title_sort oncolytic effects of reovirus in canine solid tumor cell lines
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478733/
https://www.ncbi.nlm.nih.gov/pubmed/25648933
http://dx.doi.org/10.1292/jvms.14-0570
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