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Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro

BACKGROUND: Ixeris dentata Nakai has been used for the treatment of mithridatism, calculous, indigestion, pneumonia, hepatitis, and tumors in Korea, China, and Japan. However, the effect of a water extract of Ixeris dentata (ID) and its molecular mechanism on allergic inflammation has not been eluci...

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Autores principales: Jeon, Yong-Deok, Kee, Ji-Ye, Kim, Dae-Seung, Han, Yo-Han, Kim, Sung-Hoon, Kim, Su-Jin, Um, Jae-Young, Hong, Seung-Heon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479232/
https://www.ncbi.nlm.nih.gov/pubmed/26104582
http://dx.doi.org/10.1186/s12906-015-0700-x
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author Jeon, Yong-Deok
Kee, Ji-Ye
Kim, Dae-Seung
Han, Yo-Han
Kim, Sung-Hoon
Kim, Su-Jin
Um, Jae-Young
Hong, Seung-Heon
author_facet Jeon, Yong-Deok
Kee, Ji-Ye
Kim, Dae-Seung
Han, Yo-Han
Kim, Sung-Hoon
Kim, Su-Jin
Um, Jae-Young
Hong, Seung-Heon
author_sort Jeon, Yong-Deok
collection PubMed
description BACKGROUND: Ixeris dentata Nakai has been used for the treatment of mithridatism, calculous, indigestion, pneumonia, hepatitis, and tumors in Korea, China, and Japan. However, the effect of a water extract of Ixeris dentata (ID) and its molecular mechanism on allergic inflammation has not been elucidated. In this study, we attempted to evaluate the effects of ID and its major compound caffeic acid on allergic inflammation in vivo and in vitro. METHODS: ID was applied to 2, 4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis (AD)-like skin lesion mice and immune cell infiltration, cytokine production, and the activation of mitogen-activated protein kinases (MAPKs) were investigated. Moreover, the effect of ID on compound 48/80-induced anaphylactic shock was investigated in a mouse model. The human keratinocyte cell line (HaCaT cells) and human mast cells (HMC-1) were treated with ID or caffeic acid to investigate the effects on the production of chemokines and proinflammatory cytokines and on the activation of MAPKs. RESULTS: ID inhibited the serum levels of IgE and interleukin (IL)-1β in DNFB-induced AD-like skin lesion mouse models and suppressed anaphylactic shock in the mouse models. ID and caffeic acid inhibited the production of chemokines and adhesion molecules in HaCaT cells. In addition, ID reduced the release of tumor necrosis factor-α and IL-8 via the inhibition of MAPKs phosphorylation in HMC-1 cells. CONCLUSIONS: These results suggest that ID is a potential therapeutic agent for allergic inflammatory diseases, including dermatitis.
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spelling pubmed-44792322015-06-25 Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro Jeon, Yong-Deok Kee, Ji-Ye Kim, Dae-Seung Han, Yo-Han Kim, Sung-Hoon Kim, Su-Jin Um, Jae-Young Hong, Seung-Heon BMC Complement Altern Med Research Article BACKGROUND: Ixeris dentata Nakai has been used for the treatment of mithridatism, calculous, indigestion, pneumonia, hepatitis, and tumors in Korea, China, and Japan. However, the effect of a water extract of Ixeris dentata (ID) and its molecular mechanism on allergic inflammation has not been elucidated. In this study, we attempted to evaluate the effects of ID and its major compound caffeic acid on allergic inflammation in vivo and in vitro. METHODS: ID was applied to 2, 4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis (AD)-like skin lesion mice and immune cell infiltration, cytokine production, and the activation of mitogen-activated protein kinases (MAPKs) were investigated. Moreover, the effect of ID on compound 48/80-induced anaphylactic shock was investigated in a mouse model. The human keratinocyte cell line (HaCaT cells) and human mast cells (HMC-1) were treated with ID or caffeic acid to investigate the effects on the production of chemokines and proinflammatory cytokines and on the activation of MAPKs. RESULTS: ID inhibited the serum levels of IgE and interleukin (IL)-1β in DNFB-induced AD-like skin lesion mouse models and suppressed anaphylactic shock in the mouse models. ID and caffeic acid inhibited the production of chemokines and adhesion molecules in HaCaT cells. In addition, ID reduced the release of tumor necrosis factor-α and IL-8 via the inhibition of MAPKs phosphorylation in HMC-1 cells. CONCLUSIONS: These results suggest that ID is a potential therapeutic agent for allergic inflammatory diseases, including dermatitis. BioMed Central 2015-06-24 /pmc/articles/PMC4479232/ /pubmed/26104582 http://dx.doi.org/10.1186/s12906-015-0700-x Text en © Jeon et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jeon, Yong-Deok
Kee, Ji-Ye
Kim, Dae-Seung
Han, Yo-Han
Kim, Sung-Hoon
Kim, Su-Jin
Um, Jae-Young
Hong, Seung-Heon
Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
title Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
title_full Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
title_fullStr Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
title_full_unstemmed Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
title_short Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
title_sort effects of ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479232/
https://www.ncbi.nlm.nih.gov/pubmed/26104582
http://dx.doi.org/10.1186/s12906-015-0700-x
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