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Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma
BACKGROUND: Small non-coding regulatory RNAs control cellular functions at the transcriptional and post-transcriptional levels. Oral squamous cell carcinoma is among the leading cancers in the world and the presence of cervical lymph node metastases is currently its strongest prognostic factor. In t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479233/ https://www.ncbi.nlm.nih.gov/pubmed/26104160 http://dx.doi.org/10.1186/s12920-015-0102-4 |
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author | Severino, Patricia Oliveira, Liliane Santana Andreghetto, Flávia Maziero Torres, Natalia Curioni, Otávio Cury, Patricia Maluf Toporcov, Tatiana Natasha Paschoal, Alexandre Rossi Durham, Alan Mitchell |
author_facet | Severino, Patricia Oliveira, Liliane Santana Andreghetto, Flávia Maziero Torres, Natalia Curioni, Otávio Cury, Patricia Maluf Toporcov, Tatiana Natasha Paschoal, Alexandre Rossi Durham, Alan Mitchell |
author_sort | Severino, Patricia |
collection | PubMed |
description | BACKGROUND: Small non-coding regulatory RNAs control cellular functions at the transcriptional and post-transcriptional levels. Oral squamous cell carcinoma is among the leading cancers in the world and the presence of cervical lymph node metastases is currently its strongest prognostic factor. In this work we aimed at finding small RNAs expressed in oral squamous cell carcinoma that could be associated with the presence of lymph node metastasis. METHODS: Small RNA libraries from metastatic and non-metastatic oral squamous cell carcinomas were sequenced for the identification and quantification of known small RNAs. Selected markers were validated in plasma samples. Additionally, we used in silico analysis to investigate possible new molecules, not previously described, involved in the metastatic process. RESULTS: Global expression patterns were not associated with cervical metastases. MiR-21, miR-203 and miR-205 were highly expressed throughout samples, in agreement with their role in epithelial cell biology, but disagreeing with studies correlating these molecules with cancer invasion. Eighteen microRNAs, but no other small RNA class, varied consistently between metastatic and non-metastatic samples. Nine of these microRNAs had been previously detected in human plasma, eight of which presented consistent results between tissue and plasma samples. MiR-31 and miR-130b, known to inhibit several steps in the metastatic process, were over-expressed in non-metastatic samples and the expression of miR-130b was confirmed in plasma of patients showing no metastasis. MiR-181 and miR-296 were detected in metastatic tumors and the expression of miR-296 was confirmed in plasma of patients presenting metastasis. A novel microRNA-like molecule was also associated with non-metastatic samples, potentially targeting cell-signaling mechanisms. CONCLUSIONS: We corroborate literature data on the role of small RNAs in cancer metastasis and suggest the detection of microRNAs as a tool that may assist in the evaluation of oral squamous cell carcinoma metastatic potential. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12920-015-0102-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4479233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44792332015-06-25 Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma Severino, Patricia Oliveira, Liliane Santana Andreghetto, Flávia Maziero Torres, Natalia Curioni, Otávio Cury, Patricia Maluf Toporcov, Tatiana Natasha Paschoal, Alexandre Rossi Durham, Alan Mitchell BMC Med Genomics Research Article BACKGROUND: Small non-coding regulatory RNAs control cellular functions at the transcriptional and post-transcriptional levels. Oral squamous cell carcinoma is among the leading cancers in the world and the presence of cervical lymph node metastases is currently its strongest prognostic factor. In this work we aimed at finding small RNAs expressed in oral squamous cell carcinoma that could be associated with the presence of lymph node metastasis. METHODS: Small RNA libraries from metastatic and non-metastatic oral squamous cell carcinomas were sequenced for the identification and quantification of known small RNAs. Selected markers were validated in plasma samples. Additionally, we used in silico analysis to investigate possible new molecules, not previously described, involved in the metastatic process. RESULTS: Global expression patterns were not associated with cervical metastases. MiR-21, miR-203 and miR-205 were highly expressed throughout samples, in agreement with their role in epithelial cell biology, but disagreeing with studies correlating these molecules with cancer invasion. Eighteen microRNAs, but no other small RNA class, varied consistently between metastatic and non-metastatic samples. Nine of these microRNAs had been previously detected in human plasma, eight of which presented consistent results between tissue and plasma samples. MiR-31 and miR-130b, known to inhibit several steps in the metastatic process, were over-expressed in non-metastatic samples and the expression of miR-130b was confirmed in plasma of patients showing no metastasis. MiR-181 and miR-296 were detected in metastatic tumors and the expression of miR-296 was confirmed in plasma of patients presenting metastasis. A novel microRNA-like molecule was also associated with non-metastatic samples, potentially targeting cell-signaling mechanisms. CONCLUSIONS: We corroborate literature data on the role of small RNAs in cancer metastasis and suggest the detection of microRNAs as a tool that may assist in the evaluation of oral squamous cell carcinoma metastatic potential. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12920-015-0102-4) contains supplementary material, which is available to authorized users. BioMed Central 2015-06-24 /pmc/articles/PMC4479233/ /pubmed/26104160 http://dx.doi.org/10.1186/s12920-015-0102-4 Text en © Severino et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Severino, Patricia Oliveira, Liliane Santana Andreghetto, Flávia Maziero Torres, Natalia Curioni, Otávio Cury, Patricia Maluf Toporcov, Tatiana Natasha Paschoal, Alexandre Rossi Durham, Alan Mitchell Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma |
title | Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma |
title_full | Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma |
title_fullStr | Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma |
title_full_unstemmed | Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma |
title_short | Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma |
title_sort | small rnas in metastatic and non-metastatic oral squamous cell carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479233/ https://www.ncbi.nlm.nih.gov/pubmed/26104160 http://dx.doi.org/10.1186/s12920-015-0102-4 |
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