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Effects of rhodiola crenulata on mice hearts under severe sleep apnea

BACKGROUND: The goal of this study is to determine if Rhodiola Crenulata (RC) has protective effects on mice hearts with severe sleep apnea model. METHODS: Sixty-four C57BL/6 J mice 5–6 months old were distributed into 4 groups i.e. Control group (21 % O(2), 24 h per day, 8 weeks, n = 16); Hypoxia g...

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Autores principales: Lai, Mei-Chih, Lin, Jaung-Geng, Pai, Pei-Ying, Lai, Mei-Hsin, Lin, Yueh-Min, Yeh, Yu-Lan, Cheng, Shiu-Min, Liu, Yi-fan, Huang, Chih-Yang, Lee, Shin-Da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479239/
https://www.ncbi.nlm.nih.gov/pubmed/26108210
http://dx.doi.org/10.1186/s12906-015-0698-0
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author Lai, Mei-Chih
Lin, Jaung-Geng
Pai, Pei-Ying
Lai, Mei-Hsin
Lin, Yueh-Min
Yeh, Yu-Lan
Cheng, Shiu-Min
Liu, Yi-fan
Huang, Chih-Yang
Lee, Shin-Da
author_facet Lai, Mei-Chih
Lin, Jaung-Geng
Pai, Pei-Ying
Lai, Mei-Hsin
Lin, Yueh-Min
Yeh, Yu-Lan
Cheng, Shiu-Min
Liu, Yi-fan
Huang, Chih-Yang
Lee, Shin-Da
author_sort Lai, Mei-Chih
collection PubMed
description BACKGROUND: The goal of this study is to determine if Rhodiola Crenulata (RC) has protective effects on mice hearts with severe sleep apnea model. METHODS: Sixty-four C57BL/6 J mice 5–6 months old were distributed into 4 groups i.e. Control group (21 % O(2), 24 h per day, 8 weeks, n = 16); Hypoxia group (Hypoxia: 7 % O(2) 60 s, 20 % O(2) alternating 60 s, 8 h per day, 8 weeks, n = 16); Hypoxia + 90RC and Hypoxia + 270RC group (Hypoxia for 1st 4 weeks and hypoxia pretreated 90 mg/Kg and 270 mg/Kg Rhodiola Crenulata by oral gavage per day for 2nd 4 weeks, each n = 16). Excised hearts from 4 groups of mice were analyzed for heart weight index changes using H&E staining, TUNEL-positive assays and Western Blotting protein. RESULTS: Cardiac widely dispersed TUNEL-positive apoptotic cells in mice hearts were less in Hypoxia + RC90 and Hypoxia + RC270 than those in Hypoxia. Compared with Hypoxia, the protein levels of Fas ligand, Fas death receptors, Fas-Associated Death Domain (FADD), activated caspase 8, and activated caspase 3 (Fas dependent apoptotic pathways) were decreased in Hypoxia + RC90, Hypoxia + RC270. The protein levels of Bad, Bax, t-Bid, activated caspase 9, activated caspase 3 (mitochondria dependent apoptotic pathway) were less in Hypoxia + RC90, Hypoxia + RC270 than those in hypoxia. The protein levels of Bcl2, Bcl-xL, p-Bad (Bcl2-realted anti-apoptotic pathway) and VEGF, p-PI3k, p-AKT (VEGF-related pro-survival pathway) were higher in Hypoxia + RC90, Hypoxia + RC270 than those in hypoxia. CONCLUSIONS: Our findings suggest that Rhodiola Crenulata have protective effects on chronic intermittent hypoxia-induced cardiac widely dispersed apoptosis via Fas-dependent and mitochondria-dependent apoptotic and VEGF-related pro-survival pathway.
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spelling pubmed-44792392015-06-25 Effects of rhodiola crenulata on mice hearts under severe sleep apnea Lai, Mei-Chih Lin, Jaung-Geng Pai, Pei-Ying Lai, Mei-Hsin Lin, Yueh-Min Yeh, Yu-Lan Cheng, Shiu-Min Liu, Yi-fan Huang, Chih-Yang Lee, Shin-Da BMC Complement Altern Med Research Article BACKGROUND: The goal of this study is to determine if Rhodiola Crenulata (RC) has protective effects on mice hearts with severe sleep apnea model. METHODS: Sixty-four C57BL/6 J mice 5–6 months old were distributed into 4 groups i.e. Control group (21 % O(2), 24 h per day, 8 weeks, n = 16); Hypoxia group (Hypoxia: 7 % O(2) 60 s, 20 % O(2) alternating 60 s, 8 h per day, 8 weeks, n = 16); Hypoxia + 90RC and Hypoxia + 270RC group (Hypoxia for 1st 4 weeks and hypoxia pretreated 90 mg/Kg and 270 mg/Kg Rhodiola Crenulata by oral gavage per day for 2nd 4 weeks, each n = 16). Excised hearts from 4 groups of mice were analyzed for heart weight index changes using H&E staining, TUNEL-positive assays and Western Blotting protein. RESULTS: Cardiac widely dispersed TUNEL-positive apoptotic cells in mice hearts were less in Hypoxia + RC90 and Hypoxia + RC270 than those in Hypoxia. Compared with Hypoxia, the protein levels of Fas ligand, Fas death receptors, Fas-Associated Death Domain (FADD), activated caspase 8, and activated caspase 3 (Fas dependent apoptotic pathways) were decreased in Hypoxia + RC90, Hypoxia + RC270. The protein levels of Bad, Bax, t-Bid, activated caspase 9, activated caspase 3 (mitochondria dependent apoptotic pathway) were less in Hypoxia + RC90, Hypoxia + RC270 than those in hypoxia. The protein levels of Bcl2, Bcl-xL, p-Bad (Bcl2-realted anti-apoptotic pathway) and VEGF, p-PI3k, p-AKT (VEGF-related pro-survival pathway) were higher in Hypoxia + RC90, Hypoxia + RC270 than those in hypoxia. CONCLUSIONS: Our findings suggest that Rhodiola Crenulata have protective effects on chronic intermittent hypoxia-induced cardiac widely dispersed apoptosis via Fas-dependent and mitochondria-dependent apoptotic and VEGF-related pro-survival pathway. BioMed Central 2015-06-25 /pmc/articles/PMC4479239/ /pubmed/26108210 http://dx.doi.org/10.1186/s12906-015-0698-0 Text en © Lai et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Lai, Mei-Chih
Lin, Jaung-Geng
Pai, Pei-Ying
Lai, Mei-Hsin
Lin, Yueh-Min
Yeh, Yu-Lan
Cheng, Shiu-Min
Liu, Yi-fan
Huang, Chih-Yang
Lee, Shin-Da
Effects of rhodiola crenulata on mice hearts under severe sleep apnea
title Effects of rhodiola crenulata on mice hearts under severe sleep apnea
title_full Effects of rhodiola crenulata on mice hearts under severe sleep apnea
title_fullStr Effects of rhodiola crenulata on mice hearts under severe sleep apnea
title_full_unstemmed Effects of rhodiola crenulata on mice hearts under severe sleep apnea
title_short Effects of rhodiola crenulata on mice hearts under severe sleep apnea
title_sort effects of rhodiola crenulata on mice hearts under severe sleep apnea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479239/
https://www.ncbi.nlm.nih.gov/pubmed/26108210
http://dx.doi.org/10.1186/s12906-015-0698-0
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