Cargando…
Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass
BACKGROUD: Cardiac surgery with cardiopulmonary bypass (CPB) may cause inflammatory responses, which can deteriorate the outcomes. Inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6,–8 and -10, can act as both the effector and the predictor for post-operative inflammat...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479323/ https://www.ncbi.nlm.nih.gov/pubmed/26104758 http://dx.doi.org/10.1186/s13019-015-0298-6 |
_version_ | 1782377994429399040 |
---|---|
author | Qin, Chaoyi Liu, Ruiqi Gu, Jun Li, Yajiao Qian, Hong Shi, Yingkang Meng, Wei |
author_facet | Qin, Chaoyi Liu, Ruiqi Gu, Jun Li, Yajiao Qian, Hong Shi, Yingkang Meng, Wei |
author_sort | Qin, Chaoyi |
collection | PubMed |
description | BACKGROUD: Cardiac surgery with cardiopulmonary bypass (CPB) may cause inflammatory responses, which can deteriorate the outcomes. Inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6,–8 and -10, can act as both the effector and the predictor for post-operative inflammatory responses. Plasma mitochondrial DNA (mtDNA) was found as a pro-inflammatory agent recently, which was released when cells were insulted. METHODS: In the present study, we included 38 patients undergoing coronary artery bypass graft (CABG) to analyze their perioperative plasma mtDNA and levels of inflammatory cytokines. Blood samples were collected before aortic cross-clamping (T1), at the end of CPB (T2), 6 h post-CPB (T3), 12 h post-CPB (T4), and 24 h post-CPB (T5). Rt-PCR and specific ELISA kits were used to quantify the plasma mtDNA and inflammatory cytokines, respectively. Bivariate correlations analysis was used to check the correlations between plasma mtDNA and inflammatory cytokines respectively. RESULTS: Results shown that plasma mtDNA elevated significantly at T2 and peaked at T4. Furthermore, plasma TNF-α, IL-6 and IL-8 levels significantly increased at T2 and peaked at T3 while IL-10 elevated and peaked at T2. Bivariate correlations analysis showed that the peak plasma mtDNA were positively correlated with the peak TNF-α (r = 0.677, P < 0.001), the peak IL-6 (r = 0.706, P < 0.001), the peak IL-8 (r = 0.584, P < 0.001) and the peak IL-10 (r = 0.565, P < 0.001). CONCLUSION: We found that plasma mtDNA might play a key role in CPB-induced post-operative inflammatory responses. |
format | Online Article Text |
id | pubmed-4479323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44793232015-06-25 Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass Qin, Chaoyi Liu, Ruiqi Gu, Jun Li, Yajiao Qian, Hong Shi, Yingkang Meng, Wei J Cardiothorac Surg Research Article BACKGROUD: Cardiac surgery with cardiopulmonary bypass (CPB) may cause inflammatory responses, which can deteriorate the outcomes. Inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6,–8 and -10, can act as both the effector and the predictor for post-operative inflammatory responses. Plasma mitochondrial DNA (mtDNA) was found as a pro-inflammatory agent recently, which was released when cells were insulted. METHODS: In the present study, we included 38 patients undergoing coronary artery bypass graft (CABG) to analyze their perioperative plasma mtDNA and levels of inflammatory cytokines. Blood samples were collected before aortic cross-clamping (T1), at the end of CPB (T2), 6 h post-CPB (T3), 12 h post-CPB (T4), and 24 h post-CPB (T5). Rt-PCR and specific ELISA kits were used to quantify the plasma mtDNA and inflammatory cytokines, respectively. Bivariate correlations analysis was used to check the correlations between plasma mtDNA and inflammatory cytokines respectively. RESULTS: Results shown that plasma mtDNA elevated significantly at T2 and peaked at T4. Furthermore, plasma TNF-α, IL-6 and IL-8 levels significantly increased at T2 and peaked at T3 while IL-10 elevated and peaked at T2. Bivariate correlations analysis showed that the peak plasma mtDNA were positively correlated with the peak TNF-α (r = 0.677, P < 0.001), the peak IL-6 (r = 0.706, P < 0.001), the peak IL-8 (r = 0.584, P < 0.001) and the peak IL-10 (r = 0.565, P < 0.001). CONCLUSION: We found that plasma mtDNA might play a key role in CPB-induced post-operative inflammatory responses. BioMed Central 2015-06-24 /pmc/articles/PMC4479323/ /pubmed/26104758 http://dx.doi.org/10.1186/s13019-015-0298-6 Text en © Qin et al. 2015 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Qin, Chaoyi Liu, Ruiqi Gu, Jun Li, Yajiao Qian, Hong Shi, Yingkang Meng, Wei Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass |
title | Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass |
title_full | Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass |
title_fullStr | Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass |
title_full_unstemmed | Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass |
title_short | Variation of perioperative plasma mitochondrial DNA correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass |
title_sort | variation of perioperative plasma mitochondrial dna correlate with peak inflammatory cytokines caused by cardiac surgery with cardiopulmonary bypass |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479323/ https://www.ncbi.nlm.nih.gov/pubmed/26104758 http://dx.doi.org/10.1186/s13019-015-0298-6 |
work_keys_str_mv | AT qinchaoyi variationofperioperativeplasmamitochondrialdnacorrelatewithpeakinflammatorycytokinescausedbycardiacsurgerywithcardiopulmonarybypass AT liuruiqi variationofperioperativeplasmamitochondrialdnacorrelatewithpeakinflammatorycytokinescausedbycardiacsurgerywithcardiopulmonarybypass AT gujun variationofperioperativeplasmamitochondrialdnacorrelatewithpeakinflammatorycytokinescausedbycardiacsurgerywithcardiopulmonarybypass AT liyajiao variationofperioperativeplasmamitochondrialdnacorrelatewithpeakinflammatorycytokinescausedbycardiacsurgerywithcardiopulmonarybypass AT qianhong variationofperioperativeplasmamitochondrialdnacorrelatewithpeakinflammatorycytokinescausedbycardiacsurgerywithcardiopulmonarybypass AT shiyingkang variationofperioperativeplasmamitochondrialdnacorrelatewithpeakinflammatorycytokinescausedbycardiacsurgerywithcardiopulmonarybypass AT mengwei variationofperioperativeplasmamitochondrialdnacorrelatewithpeakinflammatorycytokinescausedbycardiacsurgerywithcardiopulmonarybypass |