Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children

Although the microneutralization (MN) assay has been shown to be more sensitive than the hemagglutination inhibition (HAI) assay for the measurement of humoral immunity against influenza viruses, further evidence relating MN titres to protective efficacy against infection is needed. Serum antibodies...

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Autores principales: Verschoor, Chris P., Singh, Pardeep, Russell, Margaret L., Bowdish, Dawn M. E., Brewer, Angela, Cyr, Louis, Ward, Brian J., Loeb, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479562/
https://www.ncbi.nlm.nih.gov/pubmed/26107625
http://dx.doi.org/10.1371/journal.pone.0131531
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author Verschoor, Chris P.
Singh, Pardeep
Russell, Margaret L.
Bowdish, Dawn M. E.
Brewer, Angela
Cyr, Louis
Ward, Brian J.
Loeb, Mark
author_facet Verschoor, Chris P.
Singh, Pardeep
Russell, Margaret L.
Bowdish, Dawn M. E.
Brewer, Angela
Cyr, Louis
Ward, Brian J.
Loeb, Mark
author_sort Verschoor, Chris P.
collection PubMed
description Although the microneutralization (MN) assay has been shown to be more sensitive than the hemagglutination inhibition (HAI) assay for the measurement of humoral immunity against influenza viruses, further evidence relating MN titres to protective efficacy against infection is needed. Serum antibodies against seasonal H1N1 and H3N2 influenza were measured in children and adolescents (n = 656) by MN and hemagglutination inhibition (HAI) assays. Compared to HAI, the MN assay is more sensitive in detecting serum antibodies and estimates of protective effectiveness against PCR-confirmed infection were higher for both subtypes. Given our findings, the MN assay warrants further consideration as a formal tool for the routine evaluation of vaccine-induced antibody responses.
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spelling pubmed-44795622015-06-29 Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children Verschoor, Chris P. Singh, Pardeep Russell, Margaret L. Bowdish, Dawn M. E. Brewer, Angela Cyr, Louis Ward, Brian J. Loeb, Mark PLoS One Research Article Although the microneutralization (MN) assay has been shown to be more sensitive than the hemagglutination inhibition (HAI) assay for the measurement of humoral immunity against influenza viruses, further evidence relating MN titres to protective efficacy against infection is needed. Serum antibodies against seasonal H1N1 and H3N2 influenza were measured in children and adolescents (n = 656) by MN and hemagglutination inhibition (HAI) assays. Compared to HAI, the MN assay is more sensitive in detecting serum antibodies and estimates of protective effectiveness against PCR-confirmed infection were higher for both subtypes. Given our findings, the MN assay warrants further consideration as a formal tool for the routine evaluation of vaccine-induced antibody responses. Public Library of Science 2015-06-24 /pmc/articles/PMC4479562/ /pubmed/26107625 http://dx.doi.org/10.1371/journal.pone.0131531 Text en © 2015 Verschoor et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Verschoor, Chris P.
Singh, Pardeep
Russell, Margaret L.
Bowdish, Dawn M. E.
Brewer, Angela
Cyr, Louis
Ward, Brian J.
Loeb, Mark
Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children
title Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children
title_full Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children
title_fullStr Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children
title_full_unstemmed Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children
title_short Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children
title_sort microneutralization assay titres correlate with protection against seasonal influenza h1n1 and h3n2 in children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479562/
https://www.ncbi.nlm.nih.gov/pubmed/26107625
http://dx.doi.org/10.1371/journal.pone.0131531
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