CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer

CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced “stemness” prope...

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Autores principales: Nomura, Alice, Banerjee, Sulagna, Chugh, Rohit, Dudeja, Vikas, Yamamoto, Masato, Vickers, Selwyn M., Saluja, Ashok K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4480754/
https://www.ncbi.nlm.nih.gov/pubmed/25829252
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author Nomura, Alice
Banerjee, Sulagna
Chugh, Rohit
Dudeja, Vikas
Yamamoto, Masato
Vickers, Selwyn M.
Saluja, Ashok K.
author_facet Nomura, Alice
Banerjee, Sulagna
Chugh, Rohit
Dudeja, Vikas
Yamamoto, Masato
Vickers, Selwyn M.
Saluja, Ashok K.
author_sort Nomura, Alice
collection PubMed
description CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced “stemness” properties in MIA-PaCa2 cells along with increased tumorigenicity, tumor progression, and metastasis in vivo. Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion. EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects. This study showed that CD133 expression contributes to pancreatic cancer “stemness,” tumorigenicity, EMT induction, invasion, and metastasis.
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spelling pubmed-44807542015-06-26 CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer Nomura, Alice Banerjee, Sulagna Chugh, Rohit Dudeja, Vikas Yamamoto, Masato Vickers, Selwyn M. Saluja, Ashok K. Oncotarget Research Paper CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced “stemness” properties in MIA-PaCa2 cells along with increased tumorigenicity, tumor progression, and metastasis in vivo. Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion. EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects. This study showed that CD133 expression contributes to pancreatic cancer “stemness,” tumorigenicity, EMT induction, invasion, and metastasis. Impact Journals LLC 2015-03-16 /pmc/articles/PMC4480754/ /pubmed/25829252 Text en Copyright: © 2015 Nomura et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Nomura, Alice
Banerjee, Sulagna
Chugh, Rohit
Dudeja, Vikas
Yamamoto, Masato
Vickers, Selwyn M.
Saluja, Ashok K.
CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer
title CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer
title_full CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer
title_fullStr CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer
title_full_unstemmed CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer
title_short CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer
title_sort cd133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4480754/
https://www.ncbi.nlm.nih.gov/pubmed/25829252
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