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Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4481310/ https://www.ncbi.nlm.nih.gov/pubmed/26107953 http://dx.doi.org/10.1371/journal.pone.0100476 |
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author | Carrillo, José A. He, Yanghua Luo, Juan Menendez, Kimberly R. Tablante, Nathaniel L. Zhao, Keji Paulson, Joseph N. Li, Bichun Song, Jiuzhou |
author_facet | Carrillo, José A. He, Yanghua Luo, Juan Menendez, Kimberly R. Tablante, Nathaniel L. Zhao, Keji Paulson, Joseph N. Li, Bichun Song, Jiuzhou |
author_sort | Carrillo, José A. |
collection | PubMed |
description | In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution and annotated them regarding genes, transcription start sites (TSS) and CpG islands. We found that global DNA methylation decreased in vaccinated birds, presenting 704 hypomethylated and 451 hypermethylated DMRs, respectively. Additionally, we performed an enrichment analysis detecting gene networks, in which cancer and RNA post-transcriptional modification appeared in the first place, followed by humoral immune response, immunological disease and inflammatory disease. The top four identified canonical pathways were EIF2 signaling, regulation of EIF4 and p70S6K signaling, axonal guidance signaling and mTOR signaling, providing new insight regarding the mechanisms of ILT etiology. Lastly, the association between DNA methylation and differentially expressed genes was examined, and detected negative correlation in seventeen of the eighteen genes. |
format | Online Article Text |
id | pubmed-4481310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44813102015-06-29 Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine Carrillo, José A. He, Yanghua Luo, Juan Menendez, Kimberly R. Tablante, Nathaniel L. Zhao, Keji Paulson, Joseph N. Li, Bichun Song, Jiuzhou PLoS One Research Article In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution and annotated them regarding genes, transcription start sites (TSS) and CpG islands. We found that global DNA methylation decreased in vaccinated birds, presenting 704 hypomethylated and 451 hypermethylated DMRs, respectively. Additionally, we performed an enrichment analysis detecting gene networks, in which cancer and RNA post-transcriptional modification appeared in the first place, followed by humoral immune response, immunological disease and inflammatory disease. The top four identified canonical pathways were EIF2 signaling, regulation of EIF4 and p70S6K signaling, axonal guidance signaling and mTOR signaling, providing new insight regarding the mechanisms of ILT etiology. Lastly, the association between DNA methylation and differentially expressed genes was examined, and detected negative correlation in seventeen of the eighteen genes. Public Library of Science 2015-06-24 /pmc/articles/PMC4481310/ /pubmed/26107953 http://dx.doi.org/10.1371/journal.pone.0100476 Text en © 2015 Carrillo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Carrillo, José A. He, Yanghua Luo, Juan Menendez, Kimberly R. Tablante, Nathaniel L. Zhao, Keji Paulson, Joseph N. Li, Bichun Song, Jiuzhou Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine |
title | Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine |
title_full | Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine |
title_fullStr | Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine |
title_full_unstemmed | Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine |
title_short | Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine |
title_sort | methylome analysis in chickens immunized with infectious laryngotracheitis vaccine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4481310/ https://www.ncbi.nlm.nih.gov/pubmed/26107953 http://dx.doi.org/10.1371/journal.pone.0100476 |
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