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Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine

In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (...

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Autores principales: Carrillo, José A., He, Yanghua, Luo, Juan, Menendez, Kimberly R., Tablante, Nathaniel L., Zhao, Keji, Paulson, Joseph N., Li, Bichun, Song, Jiuzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4481310/
https://www.ncbi.nlm.nih.gov/pubmed/26107953
http://dx.doi.org/10.1371/journal.pone.0100476
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author Carrillo, José A.
He, Yanghua
Luo, Juan
Menendez, Kimberly R.
Tablante, Nathaniel L.
Zhao, Keji
Paulson, Joseph N.
Li, Bichun
Song, Jiuzhou
author_facet Carrillo, José A.
He, Yanghua
Luo, Juan
Menendez, Kimberly R.
Tablante, Nathaniel L.
Zhao, Keji
Paulson, Joseph N.
Li, Bichun
Song, Jiuzhou
author_sort Carrillo, José A.
collection PubMed
description In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution and annotated them regarding genes, transcription start sites (TSS) and CpG islands. We found that global DNA methylation decreased in vaccinated birds, presenting 704 hypomethylated and 451 hypermethylated DMRs, respectively. Additionally, we performed an enrichment analysis detecting gene networks, in which cancer and RNA post-transcriptional modification appeared in the first place, followed by humoral immune response, immunological disease and inflammatory disease. The top four identified canonical pathways were EIF2 signaling, regulation of EIF4 and p70S6K signaling, axonal guidance signaling and mTOR signaling, providing new insight regarding the mechanisms of ILT etiology. Lastly, the association between DNA methylation and differentially expressed genes was examined, and detected negative correlation in seventeen of the eighteen genes.
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spelling pubmed-44813102015-06-29 Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine Carrillo, José A. He, Yanghua Luo, Juan Menendez, Kimberly R. Tablante, Nathaniel L. Zhao, Keji Paulson, Joseph N. Li, Bichun Song, Jiuzhou PLoS One Research Article In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution and annotated them regarding genes, transcription start sites (TSS) and CpG islands. We found that global DNA methylation decreased in vaccinated birds, presenting 704 hypomethylated and 451 hypermethylated DMRs, respectively. Additionally, we performed an enrichment analysis detecting gene networks, in which cancer and RNA post-transcriptional modification appeared in the first place, followed by humoral immune response, immunological disease and inflammatory disease. The top four identified canonical pathways were EIF2 signaling, regulation of EIF4 and p70S6K signaling, axonal guidance signaling and mTOR signaling, providing new insight regarding the mechanisms of ILT etiology. Lastly, the association between DNA methylation and differentially expressed genes was examined, and detected negative correlation in seventeen of the eighteen genes. Public Library of Science 2015-06-24 /pmc/articles/PMC4481310/ /pubmed/26107953 http://dx.doi.org/10.1371/journal.pone.0100476 Text en © 2015 Carrillo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Carrillo, José A.
He, Yanghua
Luo, Juan
Menendez, Kimberly R.
Tablante, Nathaniel L.
Zhao, Keji
Paulson, Joseph N.
Li, Bichun
Song, Jiuzhou
Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
title Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
title_full Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
title_fullStr Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
title_full_unstemmed Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
title_short Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
title_sort methylome analysis in chickens immunized with infectious laryngotracheitis vaccine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4481310/
https://www.ncbi.nlm.nih.gov/pubmed/26107953
http://dx.doi.org/10.1371/journal.pone.0100476
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