Cargando…

A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices

A detailed comparison was made of the analytical features of a new Surface Plasmon Resonance (SPR) immunodevice for triazine pesticide determination with those of two other amperometric (conventional and screen-printed) immunosensors and the advantages and disadvantages of the SPR method were thorou...

Descripción completa

Detalles Bibliográficos
Autores principales: Tomassetti, Mauro, Martini, Elisabetta, Campanella, Luigi, Favero, Gabriele, Sanzó, Gabriella, Mazzei, Franco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482009/
https://www.ncbi.nlm.nih.gov/pubmed/25942643
http://dx.doi.org/10.3390/s150510255
_version_ 1782378366625644544
author Tomassetti, Mauro
Martini, Elisabetta
Campanella, Luigi
Favero, Gabriele
Sanzó, Gabriella
Mazzei, Franco
author_facet Tomassetti, Mauro
Martini, Elisabetta
Campanella, Luigi
Favero, Gabriele
Sanzó, Gabriella
Mazzei, Franco
author_sort Tomassetti, Mauro
collection PubMed
description A detailed comparison was made of the analytical features of a new Surface Plasmon Resonance (SPR) immunodevice for triazine pesticide determination with those of two other amperometric (conventional and screen-printed) immunosensors and the advantages and disadvantages of the SPR method were thoroughly investigated. For conventional amperometric and screen-printed devices, “competitive” assays were used; conversely, the SPR transduction technique allowed a “direct” measurement format to be used. As far as the main analytical data are concerned, the SPR method does not seem to offer substantial advantages. Nevertheless the measurement time is much shorter and the measurement itself much easier to perform. Lastly several applications and recovery tests were carried out on bovine milk samples, before and after spiking, to check for triazine pesticides in the samples, obtaining satisfactory results.
format Online
Article
Text
id pubmed-4482009
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-44820092015-06-29 A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices Tomassetti, Mauro Martini, Elisabetta Campanella, Luigi Favero, Gabriele Sanzó, Gabriella Mazzei, Franco Sensors (Basel) Article A detailed comparison was made of the analytical features of a new Surface Plasmon Resonance (SPR) immunodevice for triazine pesticide determination with those of two other amperometric (conventional and screen-printed) immunosensors and the advantages and disadvantages of the SPR method were thoroughly investigated. For conventional amperometric and screen-printed devices, “competitive” assays were used; conversely, the SPR transduction technique allowed a “direct” measurement format to be used. As far as the main analytical data are concerned, the SPR method does not seem to offer substantial advantages. Nevertheless the measurement time is much shorter and the measurement itself much easier to perform. Lastly several applications and recovery tests were carried out on bovine milk samples, before and after spiking, to check for triazine pesticides in the samples, obtaining satisfactory results. MDPI 2015-04-30 /pmc/articles/PMC4482009/ /pubmed/25942643 http://dx.doi.org/10.3390/s150510255 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tomassetti, Mauro
Martini, Elisabetta
Campanella, Luigi
Favero, Gabriele
Sanzó, Gabriella
Mazzei, Franco
A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices
title A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices
title_full A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices
title_fullStr A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices
title_full_unstemmed A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices
title_short A New Surface Plasmon Resonance Immunosensor for Triazine Pesticide Determination in Bovine Milk: A Comparison with Conventional Amperometric and Screen-Printed Immunodevices
title_sort new surface plasmon resonance immunosensor for triazine pesticide determination in bovine milk: a comparison with conventional amperometric and screen-printed immunodevices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482009/
https://www.ncbi.nlm.nih.gov/pubmed/25942643
http://dx.doi.org/10.3390/s150510255
work_keys_str_mv AT tomassettimauro anewsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT martinielisabetta anewsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT campanellaluigi anewsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT faverogabriele anewsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT sanzogabriella anewsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT mazzeifranco anewsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT tomassettimauro newsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT martinielisabetta newsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT campanellaluigi newsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT faverogabriele newsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT sanzogabriella newsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices
AT mazzeifranco newsurfaceplasmonresonanceimmunosensorfortriazinepesticidedeterminationinbovinemilkacomparisonwithconventionalamperometricandscreenprintedimmunodevices