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Live cell imaging of duplex siRNA intracellular trafficking

Intracellular distribution of siRNA after in vitro transfection typically depends on lipopolyplexes, which must release the siRNA into the cytosol. Here, the fate of siRNAs was monitored by FRET-based live cell imaging. Subsequent to in situ observation of uptake and release processes, this approach...

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Detalles Bibliográficos
Autores principales:	Hirsch, Markus, Helm, Mark
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	RNA
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482072/ https://www.ncbi.nlm.nih.gov/pubmed/25870407 http://dx.doi.org/10.1093/nar/gkv307

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