SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR

BACKGROUND: There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays t...

Descripción completa

Detalles Bibliográficos
Autores principales: Thompson, Christopher M., Bloom, Lee R., Ogiue-Ikeda, Mari, Machida, Kazuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482279/
https://www.ncbi.nlm.nih.gov/pubmed/26112401
http://dx.doi.org/10.1186/s12896-015-0169-1

Ejemplares similares

Ejemplares similares