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A Simple Retroelement Based Knock-Down System in Dictyostelium: Further Insights into RNA Interference Mechanisms

CHARACTERISTICS OF DIRS-1 MEDIATED KNOCK-DOWNS: We have previously shown that the most abundant Dictyostelium discoideum retroelement DIRS-1 is suppressed by RNAi mechanisms. Here we provide evidence that both inverted terminal repeats have strong promoter activity and that bidirectional expression...

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Detalles Bibliográficos
Autores principales: Friedrich, Michael, Meier, Doreen, Schuster, Isabelle, Nellen, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482531/
https://www.ncbi.nlm.nih.gov/pubmed/26110905
http://dx.doi.org/10.1371/journal.pone.0131271

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