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Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors

BACKGROUND: We analyzed aquaporin 4 and -1 expression in subependymomas, benign and slow growing brain tumors WHO grade I. Ten subependymoma cases were investigated, five of the fossa inferior and five of the fossa superior. METHODS AND RESULTS: Using immunohistochemistry, we observed different aqua...

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Autores principales: Noell, Susan, Fallier-Becker, Petra, Mack, Andreas F., Hoffmeister, Maike, Beschorner, Rudi, Ritz, Rainer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482577/
https://www.ncbi.nlm.nih.gov/pubmed/26115524
http://dx.doi.org/10.1371/journal.pone.0131367
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author Noell, Susan
Fallier-Becker, Petra
Mack, Andreas F.
Hoffmeister, Maike
Beschorner, Rudi
Ritz, Rainer
author_facet Noell, Susan
Fallier-Becker, Petra
Mack, Andreas F.
Hoffmeister, Maike
Beschorner, Rudi
Ritz, Rainer
author_sort Noell, Susan
collection PubMed
description BACKGROUND: We analyzed aquaporin 4 and -1 expression in subependymomas, benign and slow growing brain tumors WHO grade I. Ten subependymoma cases were investigated, five of the fossa inferior and five of the fossa superior. METHODS AND RESULTS: Using immunohistochemistry, we observed different aquaporin expression patterns depending on localization: aquaporin 4 and -1 were detected in infratentorial subependymomas in the entire tumor tissue. In contrast, supratentorial subependymomas revealed aquaporin 4 and -1 expression only in border areas of the tumor. PCR analyses however showed no difference in aquaporin 4 expression between all subependymomas independent of localization but at higher levels than in normal brain. In contrast, aquaporin 1 RNA levels were found to be higher only in infratentorial samples compared to supratentorial and normal brain samples. The reason for the different distribution pattern of aquaporin 4 in subependymomas still remains unclear. On the cellular level, aquaporin 4 was redistributed on the surface of the tumor cells, and in freeze fracture replicas no orthogonal arrays of particles were found. This was similar to our previous findings in malignant glioblastomas. From these studies, we know that extracellular matrix molecules within the tumor like agrin and its receptor alpha-dystroglycan are involved in forming orthogonal arrays of particles. In subependymomas neither agrin nor alpha-dystroglycan were detected around blood vessels. CONCLUSIONS: Taken together, we show in this study that in the benign subependymomas aquaporins 1 and 4 are dramatically redistributed and upregulated. We speculate that extracellular environments of infra- and supratentorial subependymomas are different and lead to different distribution patterns of aquaporin 4 and -1.
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spelling pubmed-44825772015-06-29 Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors Noell, Susan Fallier-Becker, Petra Mack, Andreas F. Hoffmeister, Maike Beschorner, Rudi Ritz, Rainer PLoS One Research Article BACKGROUND: We analyzed aquaporin 4 and -1 expression in subependymomas, benign and slow growing brain tumors WHO grade I. Ten subependymoma cases were investigated, five of the fossa inferior and five of the fossa superior. METHODS AND RESULTS: Using immunohistochemistry, we observed different aquaporin expression patterns depending on localization: aquaporin 4 and -1 were detected in infratentorial subependymomas in the entire tumor tissue. In contrast, supratentorial subependymomas revealed aquaporin 4 and -1 expression only in border areas of the tumor. PCR analyses however showed no difference in aquaporin 4 expression between all subependymomas independent of localization but at higher levels than in normal brain. In contrast, aquaporin 1 RNA levels were found to be higher only in infratentorial samples compared to supratentorial and normal brain samples. The reason for the different distribution pattern of aquaporin 4 in subependymomas still remains unclear. On the cellular level, aquaporin 4 was redistributed on the surface of the tumor cells, and in freeze fracture replicas no orthogonal arrays of particles were found. This was similar to our previous findings in malignant glioblastomas. From these studies, we know that extracellular matrix molecules within the tumor like agrin and its receptor alpha-dystroglycan are involved in forming orthogonal arrays of particles. In subependymomas neither agrin nor alpha-dystroglycan were detected around blood vessels. CONCLUSIONS: Taken together, we show in this study that in the benign subependymomas aquaporins 1 and 4 are dramatically redistributed and upregulated. We speculate that extracellular environments of infra- and supratentorial subependymomas are different and lead to different distribution patterns of aquaporin 4 and -1. Public Library of Science 2015-06-26 /pmc/articles/PMC4482577/ /pubmed/26115524 http://dx.doi.org/10.1371/journal.pone.0131367 Text en © 2015 Noell et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Noell, Susan
Fallier-Becker, Petra
Mack, Andreas F.
Hoffmeister, Maike
Beschorner, Rudi
Ritz, Rainer
Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors
title Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors
title_full Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors
title_fullStr Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors
title_full_unstemmed Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors
title_short Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors
title_sort water channels aquaporin 4 and -1 expression in subependymoma depends on the localization of the tumors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482577/
https://www.ncbi.nlm.nih.gov/pubmed/26115524
http://dx.doi.org/10.1371/journal.pone.0131367
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