Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes

Here, we introduce a new serum-free defined medium (SPM) that supports the cultivation of human pluripotent stem cells (hPSCs) on recombinant human vitronectin-N (rhVNT-N)-coated dishes after seeding with either cell clumps or single cells. With this system, there was no need for an intervening sequ...

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Detalles Bibliográficos
Autores principales: Takenaka, Chiemi, Miyajima, Hiroshi, Yoda, Yusuke, Imazato, Hideo, Yamamoto, Takako, Gomi, Shinichi, Ohshima, Yasuhiro, Kagawa, Kenichi, Sasaki, Tetsuji, Kawamata, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483150/
https://www.ncbi.nlm.nih.gov/pubmed/26115194
http://dx.doi.org/10.1371/journal.pone.0129855

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