Uncleavable Nup98–Nup96 is functional in the fission yeast Schizosaccharomyces pombe

Essential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98–Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98–N...

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Autores principales: Asakawa, Haruhiko, Mori, Chie, Ohtsuki, Chizuru, Iwamoto, Masaaki, Hiraoka, Yasushi, Haraguchi, Tokuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483485/
https://www.ncbi.nlm.nih.gov/pubmed/26137436
http://dx.doi.org/10.1016/j.fob.2015.06.004
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author Asakawa, Haruhiko
Mori, Chie
Ohtsuki, Chizuru
Iwamoto, Masaaki
Hiraoka, Yasushi
Haraguchi, Tokuko
author_facet Asakawa, Haruhiko
Mori, Chie
Ohtsuki, Chizuru
Iwamoto, Masaaki
Hiraoka, Yasushi
Haraguchi, Tokuko
author_sort Asakawa, Haruhiko
collection PubMed
description Essential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98–Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98–Nup96 joint molecule as a sole protein product of the nup189(+) gene in the fission yeast Schizosaccharomyces pombe. Cells expressing only the joint molecule were found to be viable. This result indicates that autocleavage of Nup98–Nup96 is dispensable for cell growth, at least under normal culture conditions in S. pombe.
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spelling pubmed-44834852015-07-01 Uncleavable Nup98–Nup96 is functional in the fission yeast Schizosaccharomyces pombe Asakawa, Haruhiko Mori, Chie Ohtsuki, Chizuru Iwamoto, Masaaki Hiraoka, Yasushi Haraguchi, Tokuko FEBS Open Bio Research article Essential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98–Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98–Nup96 joint molecule as a sole protein product of the nup189(+) gene in the fission yeast Schizosaccharomyces pombe. Cells expressing only the joint molecule were found to be viable. This result indicates that autocleavage of Nup98–Nup96 is dispensable for cell growth, at least under normal culture conditions in S. pombe. Elsevier 2015-06-12 /pmc/articles/PMC4483485/ /pubmed/26137436 http://dx.doi.org/10.1016/j.fob.2015.06.004 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research article
Asakawa, Haruhiko
Mori, Chie
Ohtsuki, Chizuru
Iwamoto, Masaaki
Hiraoka, Yasushi
Haraguchi, Tokuko
Uncleavable Nup98–Nup96 is functional in the fission yeast Schizosaccharomyces pombe
title Uncleavable Nup98–Nup96 is functional in the fission yeast Schizosaccharomyces pombe
title_full Uncleavable Nup98–Nup96 is functional in the fission yeast Schizosaccharomyces pombe
title_fullStr Uncleavable Nup98–Nup96 is functional in the fission yeast Schizosaccharomyces pombe
title_full_unstemmed Uncleavable Nup98–Nup96 is functional in the fission yeast Schizosaccharomyces pombe
title_short Uncleavable Nup98–Nup96 is functional in the fission yeast Schizosaccharomyces pombe
title_sort uncleavable nup98–nup96 is functional in the fission yeast schizosaccharomyces pombe
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483485/
https://www.ncbi.nlm.nih.gov/pubmed/26137436
http://dx.doi.org/10.1016/j.fob.2015.06.004
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