In vitro development of canine somatic cell nuclear transfer embryos in different culture media

The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 se...

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Autores principales: Kim, Dong-Hoon, No, Jin-Gu, Choi, Mi-Kyung, Yeom, Dong-Hyeon, Kim, Dong-Kyo, Yang, Byoung-Chul, Yoo, Jae Gyu, Kim, Min Kyu, Kim, Hong-Tea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483508/
https://www.ncbi.nlm.nih.gov/pubmed/25549216
http://dx.doi.org/10.4142/jvs.2015.16.2.233
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author Kim, Dong-Hoon
No, Jin-Gu
Choi, Mi-Kyung
Yeom, Dong-Hyeon
Kim, Dong-Kyo
Yang, Byoung-Chul
Yoo, Jae Gyu
Kim, Min Kyu
Kim, Hong-Tea
author_facet Kim, Dong-Hoon
No, Jin-Gu
Choi, Mi-Kyung
Yeom, Dong-Hyeon
Kim, Dong-Kyo
Yang, Byoung-Chul
Yoo, Jae Gyu
Kim, Min Kyu
Kim, Hong-Tea
author_sort Kim, Dong-Hoon
collection PubMed
description The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
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spelling pubmed-44835082015-06-29 In vitro development of canine somatic cell nuclear transfer embryos in different culture media Kim, Dong-Hoon No, Jin-Gu Choi, Mi-Kyung Yeom, Dong-Hyeon Kim, Dong-Kyo Yang, Byoung-Chul Yoo, Jae Gyu Kim, Min Kyu Kim, Hong-Tea J Vet Sci Short Communication The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos. The Korean Society of Veterinary Science 2015-06 2015-06-17 /pmc/articles/PMC4483508/ /pubmed/25549216 http://dx.doi.org/10.4142/jvs.2015.16.2.233 Text en © 2015 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Kim, Dong-Hoon
No, Jin-Gu
Choi, Mi-Kyung
Yeom, Dong-Hyeon
Kim, Dong-Kyo
Yang, Byoung-Chul
Yoo, Jae Gyu
Kim, Min Kyu
Kim, Hong-Tea
In vitro development of canine somatic cell nuclear transfer embryos in different culture media
title In vitro development of canine somatic cell nuclear transfer embryos in different culture media
title_full In vitro development of canine somatic cell nuclear transfer embryos in different culture media
title_fullStr In vitro development of canine somatic cell nuclear transfer embryos in different culture media
title_full_unstemmed In vitro development of canine somatic cell nuclear transfer embryos in different culture media
title_short In vitro development of canine somatic cell nuclear transfer embryos in different culture media
title_sort in vitro development of canine somatic cell nuclear transfer embryos in different culture media
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483508/
https://www.ncbi.nlm.nih.gov/pubmed/25549216
http://dx.doi.org/10.4142/jvs.2015.16.2.233
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