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Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice

This study analyzes the mechanism of influence of heat waves with strong sudden cooling on cardiovascular diseases (CVD) in ApoE−/− mice. The process of heat waves with strong sudden cooling was simulated with a TEM1880 meteorological-environment simulation chamber according to the data obtained at...

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Detalles Bibliográficos
Autores principales: Zhang, Shuyu, Kuang, Zhengzhong, Zhang, Xiakun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483669/
https://www.ncbi.nlm.nih.gov/pubmed/26016434
http://dx.doi.org/10.3390/ijerph120605743
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author Zhang, Shuyu
Kuang, Zhengzhong
Zhang, Xiakun
author_facet Zhang, Shuyu
Kuang, Zhengzhong
Zhang, Xiakun
author_sort Zhang, Shuyu
collection PubMed
description This study analyzes the mechanism of influence of heat waves with strong sudden cooling on cardiovascular diseases (CVD) in ApoE−/− mice. The process of heat waves with strong sudden cooling was simulated with a TEM1880 meteorological-environment simulation chamber according to the data obtained at 5 a.m. of 19 June 2006 to 11 p.m. of 22 June 2006. Forty-eight ApoE−/− mice were divided into six blocks based on their weight. Two mice from each block were randomly assigned to control, heat wave, temperature drop, and rewarming temperature groups. The experimental groups were transferred into the climate simulator chamber for exposure to the simulated heat wave process with strong sudden temperature drop. After 55, 59, and 75 h of exposure, the experimental groups were successively removed from the chamber to monitor physiological indicators. Blood samples were collected by decollation, and the hearts were harvested in all groups. The levels of heat stress factors (HSP60, SOD, TNF, sICAM-1, HIF-1α), cold stress factors (NE, EPI), vasoconstrictor factors (ANGII, ET-1, NO), and four items of blood lipid (TC, TG, HDL-C, and LDL-C) were measured in each ApoE−/− mouse. Results showed that the heat waves increased the levels of heat stress factors except SOD decreased, and decreased the levels of vasoconstrictor factors and blood lipid factors except TC increased. The strong sudden temperature drop in the heat wave process increased the levels of cold stress factors, vasoconstrictor factors and four blood lipid items (except the level of HDL-C which decreased) and decreased the levels of heat stress factors (except the level of SOD which increased). The analysis showed that heat waves could enhance atherosclerosis of ApoE−/− mice. The strong sudden temperature drop during the heat wave process increased the plasma concentrations of NE and ANGII, which indicates SNS activation, and resulted in increased blood pressure. NE and ANGII are vasoconstrictors involved in systemic vasoconstriction especially in the superficial areas of the body and conducive to increased blood pressure. The increase in the blood lipid levels of TG, LDL-C, TC, and LDL-C/HDL-C further aggravated CVD. This paper explored the influence mechanism of the heat waves with sudden cooling on CVD in ApoE−/− mice.
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spelling pubmed-44836692015-06-30 Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice Zhang, Shuyu Kuang, Zhengzhong Zhang, Xiakun Int J Environ Res Public Health Article This study analyzes the mechanism of influence of heat waves with strong sudden cooling on cardiovascular diseases (CVD) in ApoE−/− mice. The process of heat waves with strong sudden cooling was simulated with a TEM1880 meteorological-environment simulation chamber according to the data obtained at 5 a.m. of 19 June 2006 to 11 p.m. of 22 June 2006. Forty-eight ApoE−/− mice were divided into six blocks based on their weight. Two mice from each block were randomly assigned to control, heat wave, temperature drop, and rewarming temperature groups. The experimental groups were transferred into the climate simulator chamber for exposure to the simulated heat wave process with strong sudden temperature drop. After 55, 59, and 75 h of exposure, the experimental groups were successively removed from the chamber to monitor physiological indicators. Blood samples were collected by decollation, and the hearts were harvested in all groups. The levels of heat stress factors (HSP60, SOD, TNF, sICAM-1, HIF-1α), cold stress factors (NE, EPI), vasoconstrictor factors (ANGII, ET-1, NO), and four items of blood lipid (TC, TG, HDL-C, and LDL-C) were measured in each ApoE−/− mouse. Results showed that the heat waves increased the levels of heat stress factors except SOD decreased, and decreased the levels of vasoconstrictor factors and blood lipid factors except TC increased. The strong sudden temperature drop in the heat wave process increased the levels of cold stress factors, vasoconstrictor factors and four blood lipid items (except the level of HDL-C which decreased) and decreased the levels of heat stress factors (except the level of SOD which increased). The analysis showed that heat waves could enhance atherosclerosis of ApoE−/− mice. The strong sudden temperature drop during the heat wave process increased the plasma concentrations of NE and ANGII, which indicates SNS activation, and resulted in increased blood pressure. NE and ANGII are vasoconstrictors involved in systemic vasoconstriction especially in the superficial areas of the body and conducive to increased blood pressure. The increase in the blood lipid levels of TG, LDL-C, TC, and LDL-C/HDL-C further aggravated CVD. This paper explored the influence mechanism of the heat waves with sudden cooling on CVD in ApoE−/− mice. MDPI 2015-05-26 2015-06 /pmc/articles/PMC4483669/ /pubmed/26016434 http://dx.doi.org/10.3390/ijerph120605743 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Shuyu
Kuang, Zhengzhong
Zhang, Xiakun
Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice
title Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice
title_full Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice
title_fullStr Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice
title_full_unstemmed Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice
title_short Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice
title_sort effects of simulated heat waves with strong sudden cooling weather on apoe knockout mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483669/
https://www.ncbi.nlm.nih.gov/pubmed/26016434
http://dx.doi.org/10.3390/ijerph120605743
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