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Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study

PURPOSE: With the significance of stable adhesion of alveolar bone and peri-implant soft tissue on the surface of titanium for successful dental implantation procedure, the purpose of this study was to apply microgrooves on the titanium surface and investigate their effects on peri-implant cells and...

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Autores principales: Lee, Hyo-Jung, Lee, Jaden, Lee, Jung-Tae, Hong, Ji-Soo, Lim, Bum-Soon, Park, Hee-Jung, Kim, Young-Kwang, Kim, Tae-Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Academy of Periodontology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4485062/
https://www.ncbi.nlm.nih.gov/pubmed/26131372
http://dx.doi.org/10.5051/jpis.2015.45.3.120
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author Lee, Hyo-Jung
Lee, Jaden
Lee, Jung-Tae
Hong, Ji-Soo
Lim, Bum-Soon
Park, Hee-Jung
Kim, Young-Kwang
Kim, Tae-Il
author_facet Lee, Hyo-Jung
Lee, Jaden
Lee, Jung-Tae
Hong, Ji-Soo
Lim, Bum-Soon
Park, Hee-Jung
Kim, Young-Kwang
Kim, Tae-Il
author_sort Lee, Hyo-Jung
collection PubMed
description PURPOSE: With the significance of stable adhesion of alveolar bone and peri-implant soft tissue on the surface of titanium for successful dental implantation procedure, the purpose of this study was to apply microgrooves on the titanium surface and investigate their effects on peri-implant cells and tissues. METHODS: Three types of commercially pure titanium discs were prepared; machined-surface discs (A), sandblasted, large-grit, acid-etched (SLA)-treated discs (B), SLA and microgroove-formed discs (C). After surface topography of the discs was examined by confocal laser scanning electron microscopy, water contact angle and surface energy were measured. Human gingival fibroblasts (hGFs) and murine osteoblastic cells (MC3T3-E1) were seeded onto the titanium discs for immunofluorescence assay of adhesion proteins. Commercially pure titanium implants with microgrooves on the coronal microthreads design were inserted into the edentulous mandible of beagle dogs. After 2 weeks and 6 weeks of implant insertion, the animal subjects were euthanized to confirm peri-implant tissue healing pattern in histologic specimens. RESULTS: Group C presented the lowest water contact angle (62.89±5.66 θ), highest surface energy (45±1.2 mN/m), and highest surface roughness (Ra=22.351±2.766 µm). The expression of adhesion molecules of hGFs and MC3T30E1 cells was prominent in group C. Titanium implants with microgrooves on the coronal portion showed firm adhesion to peri-implant soft tissue. CONCLUSIONS: Microgrooves on the titanium surface promoted the adhesion of gingival fibroblasts and osteoblastic cells, as well as favorable peri-implant soft tissue sealing. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-44850622015-06-30 Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study Lee, Hyo-Jung Lee, Jaden Lee, Jung-Tae Hong, Ji-Soo Lim, Bum-Soon Park, Hee-Jung Kim, Young-Kwang Kim, Tae-Il J Periodontal Implant Sci Research Article PURPOSE: With the significance of stable adhesion of alveolar bone and peri-implant soft tissue on the surface of titanium for successful dental implantation procedure, the purpose of this study was to apply microgrooves on the titanium surface and investigate their effects on peri-implant cells and tissues. METHODS: Three types of commercially pure titanium discs were prepared; machined-surface discs (A), sandblasted, large-grit, acid-etched (SLA)-treated discs (B), SLA and microgroove-formed discs (C). After surface topography of the discs was examined by confocal laser scanning electron microscopy, water contact angle and surface energy were measured. Human gingival fibroblasts (hGFs) and murine osteoblastic cells (MC3T3-E1) were seeded onto the titanium discs for immunofluorescence assay of adhesion proteins. Commercially pure titanium implants with microgrooves on the coronal microthreads design were inserted into the edentulous mandible of beagle dogs. After 2 weeks and 6 weeks of implant insertion, the animal subjects were euthanized to confirm peri-implant tissue healing pattern in histologic specimens. RESULTS: Group C presented the lowest water contact angle (62.89±5.66 θ), highest surface energy (45±1.2 mN/m), and highest surface roughness (Ra=22.351±2.766 µm). The expression of adhesion molecules of hGFs and MC3T30E1 cells was prominent in group C. Titanium implants with microgrooves on the coronal portion showed firm adhesion to peri-implant soft tissue. CONCLUSIONS: Microgrooves on the titanium surface promoted the adhesion of gingival fibroblasts and osteoblastic cells, as well as favorable peri-implant soft tissue sealing. GRAPHICAL ABSTRACT: [Image: see text] Korean Academy of Periodontology 2015-06 2015-06-29 /pmc/articles/PMC4485062/ /pubmed/26131372 http://dx.doi.org/10.5051/jpis.2015.45.3.120 Text en Copyright © 2015 Korean Academy of Periodontology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).
spellingShingle Research Article
Lee, Hyo-Jung
Lee, Jaden
Lee, Jung-Tae
Hong, Ji-Soo
Lim, Bum-Soon
Park, Hee-Jung
Kim, Young-Kwang
Kim, Tae-Il
Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study
title Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study
title_full Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study
title_fullStr Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study
title_full_unstemmed Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study
title_short Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study
title_sort microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4485062/
https://www.ncbi.nlm.nih.gov/pubmed/26131372
http://dx.doi.org/10.5051/jpis.2015.45.3.120
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