Cargando…
Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS
As a traditional Chinese medicine, Bufei Yishen Formula (BYF) is widely used in China as an effective treatment for chronic obstructive pulmonary disease (COPD). Because of the component complexity and multiple activities of Chinese herbs, the mechanism whereby BYF affects COPD is not yet fully unde...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4485497/ https://www.ncbi.nlm.nih.gov/pubmed/26170891 http://dx.doi.org/10.1155/2015/956750 |
_version_ | 1782378790861668352 |
---|---|
author | Yang, Liping Li, Jiansheng Li, Ya Tian, Yange Li, Suyun Jiang, Suli Wang, Ying Song, Xuekun |
author_facet | Yang, Liping Li, Jiansheng Li, Ya Tian, Yange Li, Suyun Jiang, Suli Wang, Ying Song, Xuekun |
author_sort | Yang, Liping |
collection | PubMed |
description | As a traditional Chinese medicine, Bufei Yishen Formula (BYF) is widely used in China as an effective treatment for chronic obstructive pulmonary disease (COPD). Because of the component complexity and multiple activities of Chinese herbs, the mechanism whereby BYF affects COPD is not yet fully understood. Herein, pulmonary function experiments and histomorphological assessments were used to evaluate the curative effect of BYF, which showed that BYF had an effect on COPD. Additionally, a high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC QTOF/MS) metabonomics method was used to analyze the mechanism of the actions of BYF on rats with COPD induced by a combination of bacteria and smoking. Partial least squares discriminate analysis (PLS-DA) was used to screen biomarkers related to BYF treatment. Candidate biomarkers were selected and pathways analysis of these metabolites showed that three types of metabolic pathways (unsaturated fatty acid metabolism-related pathways, phenylalanine metabolism-related pathways, and phospholipid metabolism-related pathways) were associated with BYF treatment. Importantly, arachidonic acid and related metabolic pathways might be useful targets for novel COPD therapies. |
format | Online Article Text |
id | pubmed-4485497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44854972015-07-13 Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS Yang, Liping Li, Jiansheng Li, Ya Tian, Yange Li, Suyun Jiang, Suli Wang, Ying Song, Xuekun Evid Based Complement Alternat Med Research Article As a traditional Chinese medicine, Bufei Yishen Formula (BYF) is widely used in China as an effective treatment for chronic obstructive pulmonary disease (COPD). Because of the component complexity and multiple activities of Chinese herbs, the mechanism whereby BYF affects COPD is not yet fully understood. Herein, pulmonary function experiments and histomorphological assessments were used to evaluate the curative effect of BYF, which showed that BYF had an effect on COPD. Additionally, a high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC QTOF/MS) metabonomics method was used to analyze the mechanism of the actions of BYF on rats with COPD induced by a combination of bacteria and smoking. Partial least squares discriminate analysis (PLS-DA) was used to screen biomarkers related to BYF treatment. Candidate biomarkers were selected and pathways analysis of these metabolites showed that three types of metabolic pathways (unsaturated fatty acid metabolism-related pathways, phenylalanine metabolism-related pathways, and phospholipid metabolism-related pathways) were associated with BYF treatment. Importantly, arachidonic acid and related metabolic pathways might be useful targets for novel COPD therapies. Hindawi Publishing Corporation 2015 2015-06-15 /pmc/articles/PMC4485497/ /pubmed/26170891 http://dx.doi.org/10.1155/2015/956750 Text en Copyright © 2015 Liping Yang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yang, Liping Li, Jiansheng Li, Ya Tian, Yange Li, Suyun Jiang, Suli Wang, Ying Song, Xuekun Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS |
title | Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS |
title_full | Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS |
title_fullStr | Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS |
title_full_unstemmed | Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS |
title_short | Identification of Metabolites and Metabolic Pathways Related to Treatment with Bufei Yishen Formula in a Rat COPD Model Using HPLC Q-TOF/MS |
title_sort | identification of metabolites and metabolic pathways related to treatment with bufei yishen formula in a rat copd model using hplc q-tof/ms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4485497/ https://www.ncbi.nlm.nih.gov/pubmed/26170891 http://dx.doi.org/10.1155/2015/956750 |
work_keys_str_mv | AT yangliping identificationofmetabolitesandmetabolicpathwaysrelatedtotreatmentwithbufeiyishenformulainaratcopdmodelusinghplcqtofms AT lijiansheng identificationofmetabolitesandmetabolicpathwaysrelatedtotreatmentwithbufeiyishenformulainaratcopdmodelusinghplcqtofms AT liya identificationofmetabolitesandmetabolicpathwaysrelatedtotreatmentwithbufeiyishenformulainaratcopdmodelusinghplcqtofms AT tianyange identificationofmetabolitesandmetabolicpathwaysrelatedtotreatmentwithbufeiyishenformulainaratcopdmodelusinghplcqtofms AT lisuyun identificationofmetabolitesandmetabolicpathwaysrelatedtotreatmentwithbufeiyishenformulainaratcopdmodelusinghplcqtofms AT jiangsuli identificationofmetabolitesandmetabolicpathwaysrelatedtotreatmentwithbufeiyishenformulainaratcopdmodelusinghplcqtofms AT wangying identificationofmetabolitesandmetabolicpathwaysrelatedtotreatmentwithbufeiyishenformulainaratcopdmodelusinghplcqtofms AT songxuekun identificationofmetabolitesandmetabolicpathwaysrelatedtotreatmentwithbufeiyishenformulainaratcopdmodelusinghplcqtofms |