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HaloTag is an effective expression and solubilisation fusion partner for a range of fibroblast growth factors

The production of recombinant proteins such as the fibroblast growth factors (FGFs) is the key to establishing their function in cell communication. The production of recombinant FGFs in E. coli is limited, however, due to expression and solubility problems. HaloTag has been used as a fusion protein...

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Detalles Bibliográficos
Autores principales: Sun, Changye, Li, Yong, Taylor, Sarah E., Mao, Xianqing, Wilkinson, Mark C., Fernig, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4485707/
https://www.ncbi.nlm.nih.gov/pubmed/26137434
http://dx.doi.org/10.7717/peerj.1060