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A case of false mother included with 46 autosomal STR markers

BACKGROUND: For solving a maternity case, 19 autosomal short tandem repeats (STRs) were amplified using the AmpFℓSTR(®) Sinofiler(TM) kit and PowerPlex(®) 16 System. Additional 27 autosomal STR loci were analyzed using two domestic kits AGCU 21+1 and STRtyper-10G. The combined maternity index (CMI)...

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Autores principales: Li, Li, Lin, Yuan, Liu, Yan, Zhu, Ruxin, Zhao, Zhenmin, Que, Tingzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487102/
https://www.ncbi.nlm.nih.gov/pubmed/26137211
http://dx.doi.org/10.1186/s13323-015-0026-y
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author Li, Li
Lin, Yuan
Liu, Yan
Zhu, Ruxin
Zhao, Zhenmin
Que, Tingzhi
author_facet Li, Li
Lin, Yuan
Liu, Yan
Zhu, Ruxin
Zhao, Zhenmin
Que, Tingzhi
author_sort Li, Li
collection PubMed
description BACKGROUND: For solving a maternity case, 19 autosomal short tandem repeats (STRs) were amplified using the AmpFℓSTR(®) Sinofiler(TM) kit and PowerPlex(®) 16 System. Additional 27 autosomal STR loci were analyzed using two domestic kits AGCU 21+1 and STRtyper-10G. The combined maternity index (CMI) was calculated to be 3.3 × 10(13), but the putative mother denied that she had given birth to the child. In order to reach an accurate conclusion, further testing of 20 X-chromosomal short tandem repeats (X-STRs), 40 single nucleotide polymorphism (SNP) loci, and mitochondrial DNA (mtDNA) was carried out. FINDINGS: The putative mother and the boy shared at least one allele at all 46 tested autosomal STR loci. But, according to the profile data of 20 X-STR and 40 SNP markers, different genotypes at 13 X-STR loci and five SNP loci excluded maternity. Mitochondrial profiles also clearly excluded the mother as a parent of the son because they have multiple differences. It was finally found that the putative mother is the sister of the biological father. CONCLUSIONS: Different kinds of genetic markers needfully supplement the use of autosomal STR loci in case where the putative parent is suspected to be related to the true parent.
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spelling pubmed-44871022015-07-02 A case of false mother included with 46 autosomal STR markers Li, Li Lin, Yuan Liu, Yan Zhu, Ruxin Zhao, Zhenmin Que, Tingzhi Investig Genet Short Report BACKGROUND: For solving a maternity case, 19 autosomal short tandem repeats (STRs) were amplified using the AmpFℓSTR(®) Sinofiler(TM) kit and PowerPlex(®) 16 System. Additional 27 autosomal STR loci were analyzed using two domestic kits AGCU 21+1 and STRtyper-10G. The combined maternity index (CMI) was calculated to be 3.3 × 10(13), but the putative mother denied that she had given birth to the child. In order to reach an accurate conclusion, further testing of 20 X-chromosomal short tandem repeats (X-STRs), 40 single nucleotide polymorphism (SNP) loci, and mitochondrial DNA (mtDNA) was carried out. FINDINGS: The putative mother and the boy shared at least one allele at all 46 tested autosomal STR loci. But, according to the profile data of 20 X-STR and 40 SNP markers, different genotypes at 13 X-STR loci and five SNP loci excluded maternity. Mitochondrial profiles also clearly excluded the mother as a parent of the son because they have multiple differences. It was finally found that the putative mother is the sister of the biological father. CONCLUSIONS: Different kinds of genetic markers needfully supplement the use of autosomal STR loci in case where the putative parent is suspected to be related to the true parent. BioMed Central 2015-06-30 /pmc/articles/PMC4487102/ /pubmed/26137211 http://dx.doi.org/10.1186/s13323-015-0026-y Text en © Li et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Li, Li
Lin, Yuan
Liu, Yan
Zhu, Ruxin
Zhao, Zhenmin
Que, Tingzhi
A case of false mother included with 46 autosomal STR markers
title A case of false mother included with 46 autosomal STR markers
title_full A case of false mother included with 46 autosomal STR markers
title_fullStr A case of false mother included with 46 autosomal STR markers
title_full_unstemmed A case of false mother included with 46 autosomal STR markers
title_short A case of false mother included with 46 autosomal STR markers
title_sort case of false mother included with 46 autosomal str markers
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487102/
https://www.ncbi.nlm.nih.gov/pubmed/26137211
http://dx.doi.org/10.1186/s13323-015-0026-y
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