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Rapid RNA analysis of individual Caenorhabditis elegans()

Traditional RNA extraction methods rely on the use of hazardous chemicals such as phenol, chloroform, guanidinium thiocyanate to disrupt cells and inactivate RNAse simultaneously. RNA isolation from Caenorhabditis elegans presents another challenge due to its tough cuticle, therefore several repeate...

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Detalles Bibliográficos
Autores principales: Ly, Kien, Reid, Suzanne J., Snell, Russell G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487333/
https://www.ncbi.nlm.nih.gov/pubmed/26150972
http://dx.doi.org/10.1016/j.mex.2015.02.002