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Scalable microfluidics for single-cell RNA printing and sequencing

Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of...

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Detalles Bibliográficos
Autores principales: Bose, Sayantan, Wan, Zhenmao, Carr, Ambrose, Rizvi, Abbas H., Vieira, Gregory, Pe’er, Dana, Sims, Peter A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487847/
https://www.ncbi.nlm.nih.gov/pubmed/26047807
http://dx.doi.org/10.1186/s13059-015-0684-3