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The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α–NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats

Objective. To test the hypothesis that salidroside (SAL) can protect heart from exhaustive exercise-induced injury by enhancing mitochondrial respiratory function and mitochondrial biogenesis key signaling pathway PGC-1α–NRF1/NRF2 in rats. Methods. Male Sprague-Dawley rats were divided into 4 groups...

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Autores principales: Ping, Zheng, Zhang, Long-fei, Cui, Yu-juan, Chang, Yu-mei, Jiang, Cai-wu, Meng, Zhen-zhi, Xu, Peng, Liu, Hai-yan, Wang, Dong-ying, Cao, Xue-bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488012/
https://www.ncbi.nlm.nih.gov/pubmed/26167242
http://dx.doi.org/10.1155/2015/876825
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author Ping, Zheng
Zhang, Long-fei
Cui, Yu-juan
Chang, Yu-mei
Jiang, Cai-wu
Meng, Zhen-zhi
Xu, Peng
Liu, Hai-yan
Wang, Dong-ying
Cao, Xue-bin
author_facet Ping, Zheng
Zhang, Long-fei
Cui, Yu-juan
Chang, Yu-mei
Jiang, Cai-wu
Meng, Zhen-zhi
Xu, Peng
Liu, Hai-yan
Wang, Dong-ying
Cao, Xue-bin
author_sort Ping, Zheng
collection PubMed
description Objective. To test the hypothesis that salidroside (SAL) can protect heart from exhaustive exercise-induced injury by enhancing mitochondrial respiratory function and mitochondrial biogenesis key signaling pathway PGC-1α–NRF1/NRF2 in rats. Methods. Male Sprague-Dawley rats were divided into 4 groups: sedentary (C), exhaustive exercise (EE), low-dose SAL (LS), and high-dose SAL (HS). After one-time exhaustive swimming exercise, we measured the changes in cardiomyocyte ultrastructure and cardiac marker enzymes and mitochondrial electron transport system (ETS) complexes activities in situ. We also measured mitochondrial biogenesis master regulator PGC-1α and its downstream transcription factors, NRF1 and NRF2, expression at gene and protein levels. Results. Compared to C group, the EE group showed marked myocardium ultrastructure injury and decrease of mitochondrial respiratory function (P < 0.05) and protein levels of PGC-1α, NRF1, and NRF2 (P < 0.05) but a significant increase of PGC-1α, NRF1, and NRF2 genes levels (P < 0.05); compared to EE group, SAL ameliorated myocardium injury, increased mitochondrial respiratory function (P < 0.05), and elevated both gene and protein levels of PGC-1α, NRF-1, and NRF-2. Conclusion. Salidroside can protect the heart from exhaustive exercise-induced injury. It might act by improving myocardial mitochondrial respiratory function by stimulating the expression of PGC-1α–NRF1/NRF2 pathway.
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spelling pubmed-44880122015-07-12 The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α–NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats Ping, Zheng Zhang, Long-fei Cui, Yu-juan Chang, Yu-mei Jiang, Cai-wu Meng, Zhen-zhi Xu, Peng Liu, Hai-yan Wang, Dong-ying Cao, Xue-bin Oxid Med Cell Longev Research Article Objective. To test the hypothesis that salidroside (SAL) can protect heart from exhaustive exercise-induced injury by enhancing mitochondrial respiratory function and mitochondrial biogenesis key signaling pathway PGC-1α–NRF1/NRF2 in rats. Methods. Male Sprague-Dawley rats were divided into 4 groups: sedentary (C), exhaustive exercise (EE), low-dose SAL (LS), and high-dose SAL (HS). After one-time exhaustive swimming exercise, we measured the changes in cardiomyocyte ultrastructure and cardiac marker enzymes and mitochondrial electron transport system (ETS) complexes activities in situ. We also measured mitochondrial biogenesis master regulator PGC-1α and its downstream transcription factors, NRF1 and NRF2, expression at gene and protein levels. Results. Compared to C group, the EE group showed marked myocardium ultrastructure injury and decrease of mitochondrial respiratory function (P < 0.05) and protein levels of PGC-1α, NRF1, and NRF2 (P < 0.05) but a significant increase of PGC-1α, NRF1, and NRF2 genes levels (P < 0.05); compared to EE group, SAL ameliorated myocardium injury, increased mitochondrial respiratory function (P < 0.05), and elevated both gene and protein levels of PGC-1α, NRF-1, and NRF-2. Conclusion. Salidroside can protect the heart from exhaustive exercise-induced injury. It might act by improving myocardial mitochondrial respiratory function by stimulating the expression of PGC-1α–NRF1/NRF2 pathway. Hindawi Publishing Corporation 2015 2015-06-16 /pmc/articles/PMC4488012/ /pubmed/26167242 http://dx.doi.org/10.1155/2015/876825 Text en Copyright © 2015 Zheng Ping et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ping, Zheng
Zhang, Long-fei
Cui, Yu-juan
Chang, Yu-mei
Jiang, Cai-wu
Meng, Zhen-zhi
Xu, Peng
Liu, Hai-yan
Wang, Dong-ying
Cao, Xue-bin
The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α–NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats
title The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α–NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats
title_full The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α–NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats
title_fullStr The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α–NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats
title_full_unstemmed The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α–NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats
title_short The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 α–NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats
title_sort protective effects of salidroside from exhaustive exercise-induced heart injury by enhancing the pgc-1 α–nrf1/nrf2 pathway and mitochondrial respiratory function in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488012/
https://www.ncbi.nlm.nih.gov/pubmed/26167242
http://dx.doi.org/10.1155/2015/876825
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