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Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi
Microvitellogenin (mVg) is a relatively small vitellogenic protein only characterized in the eggs of the lepidopteran insects Manduca sexta and Bombyx mori. In the present study, we report a novel mVg (ApmVg) isolated from the Chinese oak silkworm Antheraea pernyi. The obtained ApmVg cDNA sequence c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488348/ https://www.ncbi.nlm.nih.gov/pubmed/26126120 http://dx.doi.org/10.1371/journal.pone.0131751 |
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author | Liu, Yanqun Chen, Miaomiao Su, Junfang Ma, Hongfang Zheng, Xixi Li, Qun Shi, Shenglin Qin, Li |
author_facet | Liu, Yanqun Chen, Miaomiao Su, Junfang Ma, Hongfang Zheng, Xixi Li, Qun Shi, Shenglin Qin, Li |
author_sort | Liu, Yanqun |
collection | PubMed |
description | Microvitellogenin (mVg) is a relatively small vitellogenic protein only characterized in the eggs of the lepidopteran insects Manduca sexta and Bombyx mori. In the present study, we report a novel mVg (ApmVg) isolated from the Chinese oak silkworm Antheraea pernyi. The obtained ApmVg cDNA sequence contains an open reading frame of 783 bp encoding a protein of 260 amino acids with a predicted molecular weight of 29.96 kDa. This gene does not contain introns. Structural analysis revealed that this protein shares putative conserved domains with the lepidopteran low-molecular weight lipoprotein, which belongs to the lipoprotein_11 superfamily. The protein sequence of ApmVg exhibits 48% sequence identity with mVg from M. sexta and 40–47% sequence identity with the 30K lipoproteins from B. mori. Phylogenetic analysis suggests that ApmVg is a novel member of the lepidopteran low-molecular weight lipoproteins. Transcriptional analysis indicated that ApmVg mRNA is mainly expressed in the fat body (both female and male) during post-diapause development of the pupal stage, and it was also detected in ovaries and spermaries in smaller amounts. RT-PCR and Western blot analyses revealed that ApmVg is synthesized by the fat body and secreted into hemolymph and ultimately accumulates in eggs. The ApmVg transcript can be detected in the fat bodies of female pupae four days after treatment with 20-hydroxyecdysone and shows an expression pattern distinct from that of vitellogenin (Vg), which is detectable throughout diapausing and in post-diapause development. ApmVg decreased dramatically during embryonic development. These results represent the first study of mVg outside M. sexta and B. mori and provide insight into the physiological role and evolution of mVgs. |
format | Online Article Text |
id | pubmed-4488348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44883482015-07-02 Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi Liu, Yanqun Chen, Miaomiao Su, Junfang Ma, Hongfang Zheng, Xixi Li, Qun Shi, Shenglin Qin, Li PLoS One Research Article Microvitellogenin (mVg) is a relatively small vitellogenic protein only characterized in the eggs of the lepidopteran insects Manduca sexta and Bombyx mori. In the present study, we report a novel mVg (ApmVg) isolated from the Chinese oak silkworm Antheraea pernyi. The obtained ApmVg cDNA sequence contains an open reading frame of 783 bp encoding a protein of 260 amino acids with a predicted molecular weight of 29.96 kDa. This gene does not contain introns. Structural analysis revealed that this protein shares putative conserved domains with the lepidopteran low-molecular weight lipoprotein, which belongs to the lipoprotein_11 superfamily. The protein sequence of ApmVg exhibits 48% sequence identity with mVg from M. sexta and 40–47% sequence identity with the 30K lipoproteins from B. mori. Phylogenetic analysis suggests that ApmVg is a novel member of the lepidopteran low-molecular weight lipoproteins. Transcriptional analysis indicated that ApmVg mRNA is mainly expressed in the fat body (both female and male) during post-diapause development of the pupal stage, and it was also detected in ovaries and spermaries in smaller amounts. RT-PCR and Western blot analyses revealed that ApmVg is synthesized by the fat body and secreted into hemolymph and ultimately accumulates in eggs. The ApmVg transcript can be detected in the fat bodies of female pupae four days after treatment with 20-hydroxyecdysone and shows an expression pattern distinct from that of vitellogenin (Vg), which is detectable throughout diapausing and in post-diapause development. ApmVg decreased dramatically during embryonic development. These results represent the first study of mVg outside M. sexta and B. mori and provide insight into the physiological role and evolution of mVgs. Public Library of Science 2015-06-30 /pmc/articles/PMC4488348/ /pubmed/26126120 http://dx.doi.org/10.1371/journal.pone.0131751 Text en © 2015 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Yanqun Chen, Miaomiao Su, Junfang Ma, Hongfang Zheng, Xixi Li, Qun Shi, Shenglin Qin, Li Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi |
title | Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi
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title_full | Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi
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title_fullStr | Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi
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title_full_unstemmed | Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi
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title_short | Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi
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title_sort | identification and characterization of a novel microvitellogenin from the chinese oak silkworm antheraea pernyi |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488348/ https://www.ncbi.nlm.nih.gov/pubmed/26126120 http://dx.doi.org/10.1371/journal.pone.0131751 |
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