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Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura

Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN83527...

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Autores principales: Lin, Xinda, Zhang, Qinhui, Wu, Zhongnan, Du, Yongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488349/
https://www.ncbi.nlm.nih.gov/pubmed/26126192
http://dx.doi.org/10.1371/journal.pone.0131407
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author Lin, Xinda
Zhang, Qinhui
Wu, Zhongnan
Du, Yongjun
author_facet Lin, Xinda
Zhang, Qinhui
Wu, Zhongnan
Du, Yongjun
author_sort Lin, Xinda
collection PubMed
description Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura, to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S. litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field.
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spelling pubmed-44883492015-07-02 Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura Lin, Xinda Zhang, Qinhui Wu, Zhongnan Du, Yongjun PLoS One Research Article Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura, to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S. litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field. Public Library of Science 2015-06-30 /pmc/articles/PMC4488349/ /pubmed/26126192 http://dx.doi.org/10.1371/journal.pone.0131407 Text en © 2015 Lin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lin, Xinda
Zhang, Qinhui
Wu, Zhongnan
Du, Yongjun
Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura
title Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura
title_full Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura
title_fullStr Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura
title_full_unstemmed Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura
title_short Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura
title_sort identification and differential expression of a candidate sex pheromone receptor in natural populations of spodoptera litura
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488349/
https://www.ncbi.nlm.nih.gov/pubmed/26126192
http://dx.doi.org/10.1371/journal.pone.0131407
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