Application of BRET to monitor ligand binding to GPCRs

Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a novel BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent...

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Autores principales: Stoddart, Leigh A, Johnstone, Elizabeth K M, Wheal, Amanda J, Goulding, Joëlle, Robers, Matthew B, Machleidt, Thomas, Wood, Keith V, Hill, Stephen J, Pfleger, Kevin D G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488387/
https://www.ncbi.nlm.nih.gov/pubmed/26030448
http://dx.doi.org/10.1038/nmeth.3398
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author Stoddart, Leigh A
Johnstone, Elizabeth K M
Wheal, Amanda J
Goulding, Joëlle
Robers, Matthew B
Machleidt, Thomas
Wood, Keith V
Hill, Stephen J
Pfleger, Kevin D G
author_facet Stoddart, Leigh A
Johnstone, Elizabeth K M
Wheal, Amanda J
Goulding, Joëlle
Robers, Matthew B
Machleidt, Thomas
Wood, Keith V
Hill, Stephen J
Pfleger, Kevin D G
author_sort Stoddart, Leigh A
collection PubMed
description Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a novel BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a novel bioluminescent protein (NanoLuc) that can be readily expressed on the N-terminus of GPCRs.
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spelling pubmed-44883872016-01-01 Application of BRET to monitor ligand binding to GPCRs Stoddart, Leigh A Johnstone, Elizabeth K M Wheal, Amanda J Goulding, Joëlle Robers, Matthew B Machleidt, Thomas Wood, Keith V Hill, Stephen J Pfleger, Kevin D G Nat Methods Article Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a novel BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a novel bioluminescent protein (NanoLuc) that can be readily expressed on the N-terminus of GPCRs. 2015-06-01 2015-07 /pmc/articles/PMC4488387/ /pubmed/26030448 http://dx.doi.org/10.1038/nmeth.3398 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Stoddart, Leigh A
Johnstone, Elizabeth K M
Wheal, Amanda J
Goulding, Joëlle
Robers, Matthew B
Machleidt, Thomas
Wood, Keith V
Hill, Stephen J
Pfleger, Kevin D G
Application of BRET to monitor ligand binding to GPCRs
title Application of BRET to monitor ligand binding to GPCRs
title_full Application of BRET to monitor ligand binding to GPCRs
title_fullStr Application of BRET to monitor ligand binding to GPCRs
title_full_unstemmed Application of BRET to monitor ligand binding to GPCRs
title_short Application of BRET to monitor ligand binding to GPCRs
title_sort application of bret to monitor ligand binding to gpcrs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488387/
https://www.ncbi.nlm.nih.gov/pubmed/26030448
http://dx.doi.org/10.1038/nmeth.3398
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