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Sexing murine embryos with an indirect immunofluorescence assay using phage antibody B9-Fab against SDM antigen
The use of serologically detectable male (SDM; also called H-Y) antigens to identify male embryos may be limited by the source of anti-SDM antibody. In the present study, novel anti-SDM B9-Fab recombinant clones (obtained by chain shuffling of an A8 original clone) were used to detect SDM antigens o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488409/ https://www.ncbi.nlm.nih.gov/pubmed/25715803 http://dx.doi.org/10.1292/jvms.14-0650 |
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author | WANG, Naidong YUAN, Anwen MA, Jun DENG, Zhibang XUE, Liqun |
author_facet | WANG, Naidong YUAN, Anwen MA, Jun DENG, Zhibang XUE, Liqun |
author_sort | WANG, Naidong |
collection | PubMed |
description | The use of serologically detectable male (SDM; also called H-Y) antigens to identify male embryos may be limited by the source of anti-SDM antibody. In the present study, novel anti-SDM B9-Fab recombinant clones (obtained by chain shuffling of an A8 original clone) were used to detect SDM antigens on murine embryos. Murine morulae and blastocysts (n=138) were flushed from the oviducts of Kunming mice and incubated with anti-SDM B9-Fab for 30 min at 37°C. With an indirect immunofluorescence assay, the membrane and inner cell mass had bright green fluorescence (presumptive males). Overall, 43.5% (60/138) were classified as presumptive males and 56.5% (78/138) as presumptive females, with 85.0 and 88.5% of these, respectively, confirmed as correct predictions (based on PCR analysis of a male-specific [Sry] sequence). We concluded that the anti-SDM B9-Fab molecule had potential for non-invasive, technically simple immunological sexing of mammalian embryos. |
format | Online Article Text |
id | pubmed-4488409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44884092015-07-06 Sexing murine embryos with an indirect immunofluorescence assay using phage antibody B9-Fab against SDM antigen WANG, Naidong YUAN, Anwen MA, Jun DENG, Zhibang XUE, Liqun J Vet Med Sci Theriogenology The use of serologically detectable male (SDM; also called H-Y) antigens to identify male embryos may be limited by the source of anti-SDM antibody. In the present study, novel anti-SDM B9-Fab recombinant clones (obtained by chain shuffling of an A8 original clone) were used to detect SDM antigens on murine embryos. Murine morulae and blastocysts (n=138) were flushed from the oviducts of Kunming mice and incubated with anti-SDM B9-Fab for 30 min at 37°C. With an indirect immunofluorescence assay, the membrane and inner cell mass had bright green fluorescence (presumptive males). Overall, 43.5% (60/138) were classified as presumptive males and 56.5% (78/138) as presumptive females, with 85.0 and 88.5% of these, respectively, confirmed as correct predictions (based on PCR analysis of a male-specific [Sry] sequence). We concluded that the anti-SDM B9-Fab molecule had potential for non-invasive, technically simple immunological sexing of mammalian embryos. The Japanese Society of Veterinary Science 2015-02-08 2015-06 /pmc/articles/PMC4488409/ /pubmed/25715803 http://dx.doi.org/10.1292/jvms.14-0650 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Theriogenology WANG, Naidong YUAN, Anwen MA, Jun DENG, Zhibang XUE, Liqun Sexing murine embryos with an indirect immunofluorescence assay using phage antibody B9-Fab against SDM antigen |
title | Sexing murine embryos with an indirect immunofluorescence assay using phage
antibody B9-Fab against SDM antigen |
title_full | Sexing murine embryos with an indirect immunofluorescence assay using phage
antibody B9-Fab against SDM antigen |
title_fullStr | Sexing murine embryos with an indirect immunofluorescence assay using phage
antibody B9-Fab against SDM antigen |
title_full_unstemmed | Sexing murine embryos with an indirect immunofluorescence assay using phage
antibody B9-Fab against SDM antigen |
title_short | Sexing murine embryos with an indirect immunofluorescence assay using phage
antibody B9-Fab against SDM antigen |
title_sort | sexing murine embryos with an indirect immunofluorescence assay using phage
antibody b9-fab against sdm antigen |
topic | Theriogenology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488409/ https://www.ncbi.nlm.nih.gov/pubmed/25715803 http://dx.doi.org/10.1292/jvms.14-0650 |
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