A Mutagenesis Assay for Reporter Gene Screening Using Partially Degenerate Oligonucleotides of the Tandems NNT and NNC

Not all proteins are tolerable to mutations. Whether a specific protein can be a mutable target is of importance in the biotechnology and pharmaceutical industry. This study reported a novel mutagenesis assay using tandem NNT and NNC oligonucleotides to test the mutability of a candidate gene. These...

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Detalles Bibliográficos
Autores principales: Xu, Huifen, Zhou, Cuilan, Zhang, Andy K., Li, Wen, Zhang, Jia, Li, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488517/
https://www.ncbi.nlm.nih.gov/pubmed/26167508
http://dx.doi.org/10.1155/2015/950873

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