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Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae

The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear. In the following study we compared the effects of such diets on redox regulation pathways during development of Atlantic cod (Gadus morhua) larv...

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Autores principales: Penglase, Samuel, Edvardsen, Rolf B., Furmanek, Tomasz, Rønnestad, Ivar, Karlsen, Ørjan, van der Meeren, Terje, Hamre, Kristin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488531/
https://www.ncbi.nlm.nih.gov/pubmed/26099546
http://dx.doi.org/10.1016/j.redox.2015.06.003
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author Penglase, Samuel
Edvardsen, Rolf B.
Furmanek, Tomasz
Rønnestad, Ivar
Karlsen, Ørjan
van der Meeren, Terje
Hamre, Kristin
author_facet Penglase, Samuel
Edvardsen, Rolf B.
Furmanek, Tomasz
Rønnestad, Ivar
Karlsen, Ørjan
van der Meeren, Terje
Hamre, Kristin
author_sort Penglase, Samuel
collection PubMed
description The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear. In the following study we compared the effects of such diets on redox regulation pathways during development of Atlantic cod (Gadus morhua) larvae. Cod larvae were fed a control diet of copepods or the typical rotifer/Artemia diet commonly used in commercial marine fish hatcheries, from first feeding until after metamorphosis. The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development. We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae. This rotifer/Artemia diet-induced differential regulation of the redox system was greatest during periods of suboptimal growth. Upregulation of the oxidative stress response transcription factor, nrf2, and NRF2 target genes in rotifer/Artemia fed larvae suggest this diet induced an NRF2-mediated oxidative stress response. Overall, the data demonstrate that nutritional intake plays a role in regulating the redox system in developing fish larvae. This may be a factor in dietary-induced differences observed in larval growth.
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spelling pubmed-44885312015-07-07 Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae Penglase, Samuel Edvardsen, Rolf B. Furmanek, Tomasz Rønnestad, Ivar Karlsen, Ørjan van der Meeren, Terje Hamre, Kristin Redox Biol Research Paper The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear. In the following study we compared the effects of such diets on redox regulation pathways during development of Atlantic cod (Gadus morhua) larvae. Cod larvae were fed a control diet of copepods or the typical rotifer/Artemia diet commonly used in commercial marine fish hatcheries, from first feeding until after metamorphosis. The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development. We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae. This rotifer/Artemia diet-induced differential regulation of the redox system was greatest during periods of suboptimal growth. Upregulation of the oxidative stress response transcription factor, nrf2, and NRF2 target genes in rotifer/Artemia fed larvae suggest this diet induced an NRF2-mediated oxidative stress response. Overall, the data demonstrate that nutritional intake plays a role in regulating the redox system in developing fish larvae. This may be a factor in dietary-induced differences observed in larval growth. Elsevier 2015-06-12 /pmc/articles/PMC4488531/ /pubmed/26099546 http://dx.doi.org/10.1016/j.redox.2015.06.003 Text en © 2015 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Research Paper
Penglase, Samuel
Edvardsen, Rolf B.
Furmanek, Tomasz
Rønnestad, Ivar
Karlsen, Ørjan
van der Meeren, Terje
Hamre, Kristin
Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae
title Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae
title_full Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae
title_fullStr Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae
title_full_unstemmed Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae
title_short Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae
title_sort diet affects the redox system in developing atlantic cod (gadus morhua) larvae
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488531/
https://www.ncbi.nlm.nih.gov/pubmed/26099546
http://dx.doi.org/10.1016/j.redox.2015.06.003
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