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MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise

MicroRNA (miRNA) are a class of non-coding, 19–25 nucleotide RNA critical for network-level regulation of gene expression. miRNA serve as paracrine signaling molecules. Using an unbiased array approach, we previously identified elevated levels of miR-224 and miR-103 to be associated with a monogenic...

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Autores principales: Bacon, Siobhán, Engelbrecht, Britta, Schmid, Jasmin, Pfeiffer, Shona, Gallagher, Ross, McCarthy, Ailbhe, Burke, Marie, Concannon, Caoimhín, Prehn, Jochen H. M., Byrne, Maria M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488671/
https://www.ncbi.nlm.nih.gov/pubmed/26110317
http://dx.doi.org/10.3390/genes6020399
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author Bacon, Siobhán
Engelbrecht, Britta
Schmid, Jasmin
Pfeiffer, Shona
Gallagher, Ross
McCarthy, Ailbhe
Burke, Marie
Concannon, Caoimhín
Prehn, Jochen H. M.
Byrne, Maria M.
author_facet Bacon, Siobhán
Engelbrecht, Britta
Schmid, Jasmin
Pfeiffer, Shona
Gallagher, Ross
McCarthy, Ailbhe
Burke, Marie
Concannon, Caoimhín
Prehn, Jochen H. M.
Byrne, Maria M.
author_sort Bacon, Siobhán
collection PubMed
description MicroRNA (miRNA) are a class of non-coding, 19–25 nucleotide RNA critical for network-level regulation of gene expression. miRNA serve as paracrine signaling molecules. Using an unbiased array approach, we previously identified elevated levels of miR-224 and miR-103 to be associated with a monogenic form of diabetes; HNF1A-MODY. miR-224 is a novel miRNA in the field of diabetes. We sought to explore the role of miR-224 as a potential biomarker in diabetes, and whether such diabetes-associated-miRNA can also be detected in the urine of patients. Absolute levels of miR-224 and miR-103 were determined in the urine of n = 144 individuals including carriers of a HNF1A mutation, participants with type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM) and normal controls. Expression levels were correlated with clinical and biochemical parameters. miR-224 was significantly elevated in the urine of carriers of a HNF1A mutation and participants with T1DM. miR-103 was highly expressed in urine across all diabetes cohorts when compared to controls. For both miR-224 and-103, we found a significant correlation between serum and urine levels (p < 0.01). We demonstrate that miRNA can be readily detected in the urine independent of clinical indices of renal dysfunction. We surmise that the differential expression levels of miR-224 in both HNF1A-MODY mutation carriers and T1DM may be an attempt to compensate for beta-cell demise.
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spelling pubmed-44886712015-07-02 MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise Bacon, Siobhán Engelbrecht, Britta Schmid, Jasmin Pfeiffer, Shona Gallagher, Ross McCarthy, Ailbhe Burke, Marie Concannon, Caoimhín Prehn, Jochen H. M. Byrne, Maria M. Genes (Basel) Article MicroRNA (miRNA) are a class of non-coding, 19–25 nucleotide RNA critical for network-level regulation of gene expression. miRNA serve as paracrine signaling molecules. Using an unbiased array approach, we previously identified elevated levels of miR-224 and miR-103 to be associated with a monogenic form of diabetes; HNF1A-MODY. miR-224 is a novel miRNA in the field of diabetes. We sought to explore the role of miR-224 as a potential biomarker in diabetes, and whether such diabetes-associated-miRNA can also be detected in the urine of patients. Absolute levels of miR-224 and miR-103 were determined in the urine of n = 144 individuals including carriers of a HNF1A mutation, participants with type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM) and normal controls. Expression levels were correlated with clinical and biochemical parameters. miR-224 was significantly elevated in the urine of carriers of a HNF1A mutation and participants with T1DM. miR-103 was highly expressed in urine across all diabetes cohorts when compared to controls. For both miR-224 and-103, we found a significant correlation between serum and urine levels (p < 0.01). We demonstrate that miRNA can be readily detected in the urine independent of clinical indices of renal dysfunction. We surmise that the differential expression levels of miR-224 in both HNF1A-MODY mutation carriers and T1DM may be an attempt to compensate for beta-cell demise. MDPI 2015-06-23 /pmc/articles/PMC4488671/ /pubmed/26110317 http://dx.doi.org/10.3390/genes6020399 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bacon, Siobhán
Engelbrecht, Britta
Schmid, Jasmin
Pfeiffer, Shona
Gallagher, Ross
McCarthy, Ailbhe
Burke, Marie
Concannon, Caoimhín
Prehn, Jochen H. M.
Byrne, Maria M.
MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise
title MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise
title_full MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise
title_fullStr MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise
title_full_unstemmed MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise
title_short MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise
title_sort microrna-224 is readily detectable in urine of individuals with diabetes mellitus and is a potential indicator of beta-cell demise
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488671/
https://www.ncbi.nlm.nih.gov/pubmed/26110317
http://dx.doi.org/10.3390/genes6020399
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